Fluorescent turn-on assay of C-type natriuretic peptide using a molecularly imprinted ratiometric fluorescent probe with
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ORIGINAL PAPER
Fluorescent turn-on assay of C-type natriuretic peptide using a molecularly imprinted ratiometric fluorescent probe with high selectivity and sensitivity Hongliang He 1,2 & Min Cao 3 & Jingwan Hu 1 & Ling Zhu 1 & Chang Su 2 & Shuhu Du 4 & Jian Yang 1 & Yulin Tang 2 & Lina Chen 4 Received: 19 May 2020 / Accepted: 29 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel molecularly imprinted ratiometric fluorescent probe was fabricated by simple sol–gel polymerization for selective and sensitive assay of C-type natriuretic peptide (CNP) in biosamples. Both the nitrobenzoxadiazole (NBD) and carbon dots (CDs) were located on the surface of silica, used as the detection signal and reference signal, respectively. For the turn-on-based probe, the fluorescence intensity of NBD could be quantitatively enhanced by CNP based on the strategy of photo-induced electron transfer (PET), while the fluorescence of CDs remained unchanged. The obtained probe exhibited excellent recognition selectivity and fast kinetics to CNP templates, and also showed good stability. The linear range of CNP determination was 5– 80 pg mL−1 with a low detection limit of 2.87 pg mL−1. Finally, the probe was successfully applied to determine CNP in human serum samples and attained high recoveries between 97.3 and 104% with precisions below 4.7%. The result indicates that the proposed method has promising potential for the assay of trace peptides in complex matrices. Keywords Ratiometric fluorescence . Molecularly imprinted polymer . C-type natriuretic peptide . Serum samples
Introduction Hongliang He and Min Cao contributed equally to this work. Hongliang He and Min Cao are co-first authors Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04583-2) contains supplementary material, which is available to authorized users. * Jian Yang [email protected] * Yulin Tang [email protected] * Lina Chen [email protected] 1
Department of Pharmacology, Nanjing Medical University, Nanjing 211166, Jiangsu, China
2
Department of Pharmacy, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, Jiangsu, China
3
Center of Pathology and Clinical Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, Jiangsu, China
4
School of Pharmacy, Nanjing Medical University, Nanjing 211166, Jiangsu, China
C-type natriuretic peptide (CNP), first isolated from porcine brain in 1990, is the most characterized member of the natriuretic peptide family with vasodilatory properties [1, 2]. It has been reported that the CNP plasma levels in chronic heart failure (CHF) patients are higher than that in healthy subjects, which can provide a complementary diagnostic test in patients with cardiovascular disease [3, 4]. Thus, the recognition and detection of CNP plasma levels are of great importance. In the past years, few methods have been developed to measure CNP in biofluids, except for the immunoassay [5–7]. Because of the extremely low con
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