Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cell
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ORIGINAL ARTICLE
Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cells Nan Yao • Bai-Qing Yang • Yu Liu • Xin-Yu Tan Cai-Ling Lu • Xiao-Hua Yuan • Xu Ma
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Received: 1 February 2010 / Accepted: 19 April 2010 / Published online: 24 August 2010 Ó Springer Science+Business Media, LLC 2010
Abstract MicroRNAs (miRNAs) regulate gene expression post-transcriptionally by interacting with the 30 untranslated regions of their target mRNAs. Previously, miRNAs have been shown to regulate genes involved in cell growth, apoptosis, and differentiation, but their role in ovarian granulosa cell follicle-stimulating hormone (FSH)stimulated steroidogenesis is unclear. Here we show that expression of 31 miRNAs is altered during FSH-mediated progesterone secretion of cultured granulosa cells. Specifically, 12 h after FSH treatment, miRNAs mir-29a and mir30d were significantly down-regulated. However, their expression increased after 48 h. Bioinformatic analysis used to predict potential targets of mir-29a and mir-30d revealed a wide array of potential mRNA target genes, including those encoding genes involved in multiple Bai-Qing Yang has contributed equally to this work.
Electronic supplementary material The online version of this article (doi:10.1007/s12020-010-9345-1) contains supplementary material, which is available to authorized users. N. Yao B.-Q. Yang X.-H. Yuan X. Ma (&) Graduate School of Peking Union Medical College, Beijing, China e-mail: [email protected] N. Yao B.-Q. Yang C.-L. Lu (&) X.-H. Yuan X. Ma Department of Genetics, National Research Institute for Family Planning, Beijing, China e-mail: [email protected] Y. Liu Department of Etiology and Carcinogenesis, Cancer Institute (Hospital), Peking Union Medical College, Beijing, China X.-Y. Tan Key Lab Genome Science & Informat, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China
signaling pathways. Taken together, our results pointed to a novel mechanism for the pleiotropic effects of FSH. Keywords Granulosa cells FSH miRNA Progesterone
Introduction The primary trigger of immature preantral follicle development into a preovulatory follicle is follicle-stimulating hormone (FSH) stimulation of granulosa cell differentiation [1]. FSH controls the development of granulosa cells during folliculogenesis by stimulating their proliferation and differentiation, and by promoting the formation of the follicular antrum [2]. In particular, FSH enhances the biosynthesis of female sex hormones estradiol and progesterone, which control the estrous cycle and reproduction [3–5]. FSH transcriptionally regulates multiple granulosa cell genes during FSH-induced steroidogenesis, including decay accelerating factor GPI-form precursor (DAF), insulin-like growth factor binding protein 3 (IGFBP3), steroidogenic acute regulatory protein (StAR), bone morphogenetic proteins (BMPs) [6–9]. Previously, we demonstrated that the expression of some microRNAs (miRNAs) (let-7a, mir-125b and
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