Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats
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Microbial Cell Factories Open Access
RESEARCH
Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats Sihan Li1,2†, Youcheng He1†, Haiou Zhang1, Rong Zheng1, Ruoying Xu1, Qihong Liu1, Shuihua Tang1, Xiao Ke1* and Minghan Huang1*
Abstract In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the intestinal flora. We found that the relative abundance of Firmicutes with constipation was significantly higher accounted for 86.7%, while the gut microbiota was significantly changed after taking a certain dose of Chinese medicine, greatly increased the relative abundance of Lactobacillus accounted for 23.1%, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation. Keywords: Constipation, Traditional Chinese medicine, Gut microbiota, Lactobacillus, Firmicutes Introduction Constipation is a common clinical gastrointestinal disease, mainly manifested as irregular defecation, difficulty in defecation. The total incidence rate of the disease is around 16% [1]. As the disease spectrum changes, Chronic constipation has gradually become a common problem affecting children’s physical and mental health [2, 3]. According to a US survey, constipation plagues in healthy people, and constipation are also associated with colon cancer, heart, brain vessels, and the elderly dementia and so on [4]. Meanwhile, long term constipation can lead to intestinal wall damage, hemorrhoids and anal fissure, and the accumulation of enterotoxin can also *Correspondence: [email protected]; [email protected] † Sihan Li and Youcheng He contributed equally to this work 1 Department of Gastroenterology, The Second People’s Hospital affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 353003, China Full list of author information is available at the end of the article
easily lead to cytopathic changes, thus leading to colorectal cancer [5]; elderly constipation patients with cardiovascular and cerebrovascular diseases can induce acute myocardial infarction and other serious diseases due to excessive abdominal pressure due to forced defecation. In addition, constipation has an important relationship with hepatic encephalopathy, breast disease, Alzheimer’s disease, women’s dysmenorrhea, urinary tract infection and other diseases [4]. The pathogenesis of chronic constipation mainly includes the reduction of colonic contraction, the dysfunction of pelvic floor and defecation, the abnormality of intestinal neurons and neurotransmitters, and the a
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