Fresh and in vitro osteodifferentiated human amniotic membrane, alone or associated with an additional scaffold, does no
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Fresh and in vitro osteodifferentiated human amniotic membrane, alone or associated with an additional scaffold, does not induce ectopic bone formation in Balb/c mice Romain Laurent . Aure´lie Nallet . Benoit de Billy . Laurent Obert . Laurence Nicod . Christophe Meyer . Pierre Layrolle . Narcisse Zwetyenga . Florelle Gindraux
Received: 22 March 2016 / Accepted: 8 December 2016 Ó Springer Science+Business Media Dordrecht 2016
Abstract The human amniotic membrane (hAM) has been successfully used as a natural carrier containing amniotic mesenchymal stromal cells, epithelial cells and growth factors. It has a little or no immunogenicity, and possesses useful anti-microbial, anti-inflammatory, anti-fibrotic and analgesic properties. It has been used for many years in several indications for soft tissue repair. We previously reported that hAM represents a natural and preformed sheet containing highly potent stem cells, and could thus be used for bone repair. Indeed, native hAM possesses pre-osteoblastic potential that can easily be stimulated, even as far as mineralization, by means of in vitro osteogenic culture. However, cell culture induces damage to the tissue, as well as to cell phenotype and function. The aim of this study was to
evaluate new bone formation by fresh and in vitro osteodifferentiated hAM, alone or associated with an additional scaffold presenting osteoinductive properties. Moreover, we also aimed to determine the effect of in vitro hAM pre-osteodifferentiation on its in vivo biocompatibility/tissue degradation. Results showed that neither fresh nor osteodifferentiated hAM induced ectopic bone formation, whether or not it was associated with the osteoinductive scaffold. Secondly, fresh and osteodifferentiated hAM presented similar in vivo tissue degradation, suggesting that in vitro hAM pre-osteodifferentiation did not influence its in vivo biocompatibility.
R. Laurent B. de Billy Paediatric Surgery Service, University Hospital of Besancon, Besanc¸on, France
C. Meyer Maxillofacial Surgery Service, University Hospital of Besancon, Besanc¸on, France
A. Nallet Novotec, Lyon, France
P. Layrolle Inserm U957, Laboratory for Pathophysiology of Bone Resorption, Faculty of Medicine, University of Nantes, Nantes, France
B. de Billy L. Obert L. Nicod C. Meyer F. Gindraux Nanomedicine Lab, Imagery and Therapeutics (EA 4662), SFR FED 4234, University of Franche-Comte´, Besanc¸on, France L. Obert F. Gindraux (&) Orthopaedic, Traumatology and Plastic Surgery Service, University Hospital of Besancon, Besanc¸on, France e-mail: [email protected]
Keywords Mice Osteogenic potential Immune reaction Bone Ectopic Biocompatibility
N. Zwetyenga Department of Maxillofacial Surgery, Plastic Reconstructive and Aesthetic Surgery, Hand Surgery, University Hospital of Dijon, Dijon, France
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Cell Tissue Bank
Introduction The human amniotic membrane (hAM) is the innermost layer of the foetal membrane, and is a thin, highly flexible, translucent, and semipermeable membrane possessing inte
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