FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae
- PDF / 2,127,289 Bytes
- 8 Pages / 547.087 x 737.008 pts Page_size
- 75 Downloads / 203 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL RESEARCH PAPER
FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae Gowsalya Ramachandran . Ravi Chidambaram . Vasanthi Nachiappan
Received: 28 April 2020 / Accepted: 8 September 2020 Ó Springer Nature B.V. 2020
Abstract Objectives To elucidate the role of FSH1 (family of serine hydrolase) in lipid homeostasis. Results Proteins in various species containing alpha/beta hydrolase domain are known to be involved in lipid metabolism. In silico analysis of the FSH1 gene in Saccharomyces cerevisiae revealed the presence of alpha/beta hydrolase domain (ABHD) and a lipase motif (GXSXG), however its function in lipid metabolism remained elusive. The overexpression of FSH1 in WT and fsh1D cells showed a significant reduction in the cellular phospholipid levels and an increase in the triacylglycerol levels and lipid droplet (LD) number. Furthermore, the purified recombinant protein Fsh1p was identified as a lysophospholipase that specifically acts on lysophosphatidylserine (LPS) and impacts the lipid homeostasis in S. cerevisiae. Conclusions These results depicted that Fsh1p has a role on lipid homeostasis and is a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS).
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10529-020-03004-x) contains supplementary material, which is available to authorized users. G. Ramachandran R. Chidambaram V. Nachiappan (&) Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India e-mail: [email protected]
Keywords FSH1 Phospholipase FFA Lipid droplets Phospholipids
Introduction Saccharomyces cerevisiae is a model system to study cellular homeostasis and lipid metabolism efficiently. Lipids are essential in cellular functions (Santos and Riezman 2012), and phospholipids are involved in membrane formation, intracellular membrane trafficking, signal transduction, molecular chaperons, cell division and serve as precursors for the synthesis of macromolecule (Rajakumari and Daum 2010a, b). Phospholipases play a vital role in lipid homeostasis and remodeling (De Smet et al. 2013). The phospholipases B isoforms (PLB1, PLB2, and PLB3) hydrolyze the phospholipids at both sn-1 and sn-2 positions and release free fatty acid (FFA). The PLB1 encodes lysophospholipases (Lee et al. 1994); deacylation of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in yeast and is found to be Plb1p localized in endoplasmic reticulum (ER), plasma membrane, and vesicles (Fyrst et al. 1999). The Plb2p has broader substrate specificity (Fyrst et al. 1999) and Plb3p deacylates phosphatidylinositol (PI) and phosphatidylserine (PS) (Lee et al. 1994; Merkel et al. 1999, 2005). The NTE1 encoded phospholipase B cleaves PC at both sn-1 and sn-2 positions and is an
123
Biotechnol Lett
integral ER membrane protein (Zaccheo et al. 2004; Murray and McMaster 2005, 2007; Kuwabara et al. 1988; Saito et al. 1991). The ph
Data Loading...
