Mitochondrial-nuclear coadaptation revealed through mtDNA replacements in Saccharomyces cerevisiae
- PDF / 1,671,672 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 6 Downloads / 198 Views
RESEARCH ARTICLE
Open Access
Mitochondrial-nuclear coadaptation revealed through mtDNA replacements in Saccharomyces cerevisiae Tuc H. M. Nguyen1, Sargunvir Sondhi1, Andrew Ziesel1, Swati Paliwal2 and Heather L. Fiumera1*
Abstract Background: Mitochondrial function requires numerous genetic interactions between mitochondrial- and nuclearencoded genes. While selection for optimal mitonuclear interactions should result in coevolution between both genomes, evidence for mitonuclear coadaptation is challenging to document. Genetic models where mitonuclear interactions can be explored are needed. Results: We systematically exchanged mtDNAs between 15 Saccharomyces cerevisiae isolates from a variety of ecological niches to create 225 unique mitochondrial-nuclear genotypes. Analysis of phenotypic profiles confirmed that environmentally-sensitive interactions between mitochondrial and nuclear genotype contributed to growth differences. Exchanges of mtDNAs between strains of the same or different clades were just as likely to demonstrate mitonuclear epistasis although epistatic effect sizes increased with genetic distances. Strains with their original mtDNAs were more fit than strains with synthetic mitonuclear combinations when grown in media that resembled isolation habitats. Conclusions: This study shows that natural variation in mitonuclear interactions contributes to fitness landscapes. Multiple examples of coadapted mitochondrial-nuclear genotypes suggest that selection for mitonuclear interactions may play a role in helping yeasts adapt to novel environments and promote coevolution. Keywords: Mitonuclear, Cytonuclear incompatibilities, Mt-n, Mitochondrial-nuclear, Coevolution, Coadaptation, G × G, G × G × E, Saccharomyces
Background Mitochondria are the cytoplasmic organelles that power eukaryotic life. Their functions in energy production, nutrient and redox sensing and signaling pathways are governed by both the mitochondrial and nuclear genomes. Known genetic interactions between these distinct organellar DNAs are required for basic mitochondrial functions including mitochondrial DNA (mtDNA) replication and repair, transcription and translation of mitochondrial genes, assembly and function of the respiratory chain * Correspondence: [email protected] 1 Department of Biological Sciences, Binghamton University, Binghamton, NY, USA Full list of author information is available at the end of the article
complexes and creation of a mitochondrial membrane potential that drives energy production and maintains mitochondrial homeostasis [1, 2]. These genetic interactions have shaped the evolution of both genomes [3–5]. As mtDNAs diverge in different populations of a species, selection should favor coevolved nuclear alleles that help maintain important mitochondrial activities. During hybridization, these coevolved mitochondrial-nuclear (mitonuclear) allele pairs may be disrupted resulting in reduced fitness in the hybrids and/or F2 progeny in a type of Bateson-Dobzhansky-Muller (BDM) incompatibility that leads to r
Data Loading...
