FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
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APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY
FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides Zsuzsa Szabó 1,2 & Klaudia Pákozdi 1,3 & Katalin Murvai 1 & Tünde Pusztahelyi 4 & Ádám Kecskeméti 5 & Attila Gáspár 5 & Antonio F. Logrieco 6 & Tamás Emri 1 & Attila L. Ádám 7 & Éva Leiter 1 & László Hornok 8 & István Pócsi 1 Received: 27 February 2020 / Revised: 22 May 2020 / Accepted: 1 June 2020 # The Author(s) 2020
Abstract FvatfA from the maize pathogen Fusarium verticillioides putatively encodes the Aspergillus nidulans AtfA and Schizasaccharomyces pombe Atf1 orthologous bZIP-type transcription factor, FvAtfA. In this study, a ΔFvatfA deletion mutant was constructed and then genetically complemented with the fully functional FvatfA gene. Comparing phenotypic features of the wild-type parental, the deletion mutant and the restored strains shed light on the versatile regulatory functions played by FvAtfA in (i) the maintenance of vegetative growth on Czapek-Dox and Potato Dextrose agars and invasive growth on unwounded tomato fruits, (ii) the preservation of conidiospore yield and size, (iii) the orchestration of oxidative (H2O2, menadione sodium bisulphite) and cell wall integrity (Congo Red) stress defences and (iv) the regulation of mycotoxin (fumonisins) and pigment (bikaverin, carotenoid) productions. Expression of selected biosynthetic genes both in the fumonisin (fum1, fum8) and the carotenoid (carRA, carB) pathways were down-regulated in the ΔFvatfA strain resulting in defected fumonisin production and considerably decreased carotenoid yields. The expression of bik1, encoding the polyketide synthase needed in bikaverin biosynthesis, was not up-regulated by the deletion of FvatfA meanwhile the ΔFvatfA strain produced approximately ten times more bikaverin than the wild-type or the genetically complemented strains. The abolishment of fumonisin production of the ΔFvatfA strain may lead to the development of new-type, biology-based mycotoxin control strategies. The novel information gained on the regulation of pigment production by this fungus can be interesting for experts working on new, Fusarium-based biomass and pigment production technologies. Key points • FvatfA regulates vegetative and invasive growths of F. verticillioides. • FvatfA also orchestrates oxidative and cell wall integrity stress defenses. • The ΔFvatfA mutant was deficient in fumonisin production. • FvatfA deletion resulted in decreased carotenoid and increased bikaverin yields.
Keywords Vegetative growth . Invasive growth . Conidiogenesis . Oxidative stress . Cell wall integrity stress . Mycotoxin production . Pigment production Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10717-6) contains supplementary material, which is available to authorized users. * István Pócsi [email protected]
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Central Laboratory of Agricultural and Food Products, Faculty of Agricultural and Food Sciences and Environmental Man
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