Gene editing applications to modulate crop flowering time and seed dormancy
- PDF / 671,441 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 208 Views
aBIOTECH
REVIEW
Gene editing applications to modulate crop flowering time and seed dormancy Olena Kishchenko1,2,3 , Yuzhen Zhou1,2 , Satyvaldy Jatayev4 Yuri Shavrukov5 , Nikolai Borisjuk1,2& 1
2
3 4 5
,
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huai’an, China Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai’an, China Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Kiev, Ukraine Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, Australia
Received: 29 June 2020 / Accepted: 10 October 2020
Abstract
Gene editing technologies such as CRISPR/Cas9 have been used to improve many agricultural traits, from disease resistance to grain quality. Now, emerging research has used CRISPR/Cas9 and other gene editing technologies to target plant reproduction, including major areas such as flowering time and seed dormancy. Traits related to these areas have important implications for agriculture, as manipulation of flowering time has multiple applications, including tailoring crops for regional adaptation and improving yield. Moreover, understanding seed dormancy will enable approaches to improve germination upon planting and prevent pre-harvest sprouting. Here, we summarize trends and recent advances in using gene editing to gain a better understanding of plant reproduction and apply the resulting information for crop improvement.
Keywords Genome editing, CRISPR/Cas9, Flowering time, Florigen, Seed dormancy
INTRODUCTION Supplementing traditional breeding and selection with new genome manipulation technologies, such as plant transformation and (more recently) targeted genome editing, could substantially accelerate crop improvement (Borisjuk et al. 2019; Chen et al. 2019). Genome editing using specific targeted nucleases is a relatively young, burgeoning technology that is rapidly becoming an integral part of research and development in many areas of life science. Boosted by the advent of CRISPR/ Cas9 nuclease systems based on target recognition by RNA:DNA complementarity, gene editing has had a huge & Correspondence: [email protected] (N. Borisjuk)
impact on plant biology in less than 10 years, as it was readily adopted to introduce specific genetic changes in plant genomes in experiments that helped resolve difficult scientific questions and improved important traits in major agricultural crops. The rapid adoption of this technology for basic and applied research on the world’s most important crops is well illustrated by the number of NCBI-registered publications yielded by searches using the crop’s name “AND CRISPR” as a query; as of September 2020, this query yielded: 526 hits for rice (Oryza sativa), 127 for wheat (Triticum aestivum), 156 for maize (Zea mays), and 376 for Arabidopsis thaliana. A variety of co
Data Loading...
