Genetic and epigenetic variations in barley ( Hordeum vulgare L.) using SSR and MSAP approaches
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RESEARCH ARTICLE
Genetic and epigenetic variations in barley (Hordeum vulgare L.) using SSR and MSAP approaches Bo Li . Qiang Gong . Wei Hua . Gonghai Chen . Wenying Zhang . Yanhao Xu . Chengdao Li
Received: 5 February 2020 / Accepted: 16 September 2020 Ó Springer Nature B.V. 2020
Abstract Barley (Hordeum vulgare L.) is the fourth largest cereal crop in the world with extensive adaptability in diverse environments. There is increasing evidence that epigenetics contributes to plant evolution and adaptation. However, epigenetic variation in barley and their correlations with genetic variation remains largely unknown. In this study, we investigated epigenetic and genetic diversity in 48 Tibetan wild distribution (TWD) barley accessions, 24 Chinese cultivars (CC), and 24 foreign cultivars (FC) using DNA methylation-sensitive amplified polymorphism and simple sequence repeats. We found a relatively high level of epigenetic (I = 0.639 and h = 0.450) and genetic (I = 0.637 and h = 0.446)
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-01019-x) contains supplementary material, which is available to authorized users. B. Li Q. Gong W. Zhang Y. Xu (&) Hubei Key Laboratory of Waterlogging Disaster and Agriculture Use of Wetland/Hubei Collaborative Innovation Centre for Grain, Industry/Engineering Research Center of Ecology and Agriculture Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, Hubei, China e-mail: [email protected]
diversity in barley, and a significant correlation between epigenetic and genetic variation in barley (R2 = 0.160, P \ 0.001). No differences in genetic variation were observed among TWD, CC and FC populations. However, the TWD population had significantly higher epigenetic diversity (I = 0.607 and h = 0.424, P \ 0.001) than both cultivated populations (FC: I = 0.584 and h = 0.402; CC: I = 0.544 and h = 0.372). Also, the TWD population had significantly higher average hemi-methylation (20.35%) and full-methylation (25.37%) levels than both cultivated populations (FC: 16.13% and 20.98%, respectively; CC: 15.98% and 18.95%, respectively). This study has provided valuable information on DNA methylation variation across diverse barley accessions and indicated that epigenetic variations might be another factor contributing to barley diversity.
G. Chen Jingzhou Academy of Agricultural Sciences, Jingzhou 434000, Hubei, China C. Li (&) Western Barley Genetics Alliance, Murdoch University, Murdoch, WA 6150, Australia e-mail: [email protected]
W. Hua Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
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Genet Resour Crop Evol
Keywords Barley
Epi/genetic diversity SSR MSAP
Introduction Barley (Hordeum vulgare L.) is cultivated globally across a wide range of environments from QinghaiTibet Plateau to sub-sea level alluvial plain. It is used for feed, brewing malts, and human consumption (Ma
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