Genetic relationships among Iranian and exotic safflower using microsatellite markers
- PDF / 393,457 Bytes
- 7 Pages / 612 x 811 pts Page_size
- 101 Downloads / 182 Views
J. Crop Sci. Biotech. 2017 (September) 20 (3) : 159 ~ 165 DOI No. 10.1007/s12892-017-0001-0 RESEARCH ARTICLE
Genetic Relationships among Iranian and Exotic Safflower Using Microsatellite Markers Moslem Bahmankar1*, Daryoosh Ahmadi Nabati2, Masood Dehdari3 1
Department of Agronomy and Plant Breeding, College of Aburaihan, University of Tehran, Tehran, Iran Department of Agronomy and Plant Breeding, College of Agriculture, University of Shahid Chamran Ahvaz, Ahvaz, Iran 3 Department of Agronomy and Plant Breeding, College of Agriculture, University of Yasouj, Yasouj, Iran 2
Received: January 02, 2017 / Revised: April 10, 2017 / Accepted: June 24, 2017 Ⓒ Korean Society of Crop Science and Springer 2017
Abstract Safflower (Carthamus tinctorius L.) is mainly grown as an oil seed crop in the semiarid and temperate regions of the world. In this study, microsatellite markers were used to evaluate and characterize genetic relationships among 20 safflower genotypes including six Iranian and 14 exotic ones. Nine primer pairs generated clear scoring bands and yielded 22 alleles ranging from 100 to 400 bp with 2-4 alleles per locus. Polymorphic information content (PIC) values ranged from 0.11 to 0.62 with an average of 0.33. The Dice similarity coefficients among the genotypes ranged from 0.06 (between Soviet Union 2 and USA) to 0.95 (between Local Isfahan and Indian 2), indicating a relatively high genetic variation among the safflower genotypes studied. Both cluster and principal components analysis (PCA) clearly classified safflower genotypes into four groups. Results indicated that in most cases safflower genotypes were divided into the groups consistent with their country of origin. Clustering some foreign genotypes into the Iranian group was due to their similar genetic basis and it was suggested that Iran may be the origin of these. The findings suggested that microsatellite markers could be efficiently utilized to assess genetic diversity and relationships among safflower genotypes. Key words : Safflower, genetic diversity, microsatellite markers, cluster analysis
Introduction Safflower (Carthamus tinctorious L.) is an annual medicinal and oilseed crop which is diploid and has 2n =2x =24 chromosomes (Ashri 1975; Bowles et al. 2010; Jaradat and Shahid 2006). The origins of Safflower are Mediterranean, Middle East, and Iran (Ashri 1975; Bowles et al. 2010). Traditionally, it was cultivated for its flowers, which were used as dye sources for coloring food and textiles as well as medicinal purposes. Nowadays, safflower is mainly grown for its seed oil and other industrial applications such as biofuel (Bahmankar et al. 2016; Hamdan et al. 2011). In breeding programs, it would be highly useful and cost effective to assess genotypes using molecular markers. Since molecular markers revealed higher polymorphism, an independent environment and plant growth stage, so they could be employed to study genetic diversity (Golkar et al. 2011; Li et Moslem Bahmankar () Email: [email protected] Tel: +989366379582 Fax: +98213604
Data Loading...
