Genetic variation in mutants of black gram ( Vigna mungo (L.) Hepper) evaluated by RAPD markers
- PDF / 120,976 Bytes
- 6 Pages / 609.451 x 793.701 pts Page_size
- 40 Downloads / 200 Views
J. Crop Sci. Biotech. 2010 (March) 13 (1) : 1 ~ 6 DOI No. 10.1007/s12892-009-0132-6 RESEARCH ARTICLE
Genetic Variation in Mutants of Black Gram (Vigna mungo (L.) Hepper) Evaluated by RAPD Markers D. Arulbalachandran1*, L. Mullainathan1, S. Karthigayan2, S.T. Somasundaram2, S. Velu1. Department of Botany1, CAS in Marine Biology2, Annamalai University, Annamalainagar - 608 002, TN, India Received: October 13, 2009 / Revised: December 6, 2009 / Accepted: December 11, 2009 Ⓒ Korean Society of Crop Science and Springer 2009
Abstract Genetic variation among four mutants with parent of black gram (high seed protein, tall, bushy, and dwarf mutants) was investigated. The mutants were generated with physical gamma rays and chemical ethyl methane sulphonate. These mutants had some advantages like high protein content, lodging resistance, and high seed yield compared with parent cultivars (cvs.). The objective of the present investigation is to find genetic variation in mutants of black gram with their parents. The genetic variation was evaluated with 20 random primers, generated total 202 fragments scored with 58 polymorphic alleles, and the average was 10.1 alleles per locus and a range of 1-9 alleles. The average polymorphic rates were 38.37 among the mutants and parents through the 20 primers. Primers OPA-14 and OPI-04 revealed 35% of DNA polymorphism in this investigation. The genetic distance (GD) among the genotypes was 0.19 suggesting a significant degree of genetic diversity. The five genotypes were used to construct a dendrogram based on the similarity matrix, revealing a genetic distance varying from 0.600 to 0.725. This variation was due to the mutation induced by gamma rays and ethyl methane sulphonate. Further research is needed to analyze the sequence and expression of these mutated genes and to develop and improve black gram via genetic engineering to evaluate their use. Key words: ethyl methanesulphonate, gamma rays, black gram, genetic polymorphism, mutants, random amplified polymorphic DNA
Introduction Black gram (Vigna mungo (L.) Hepper) is a tropical crop belonging to the Asiatic Vigna species. The study of genetic diversity in various species of the genus Vigna using a DNAbased marker system is limited to a large extent (Amadou et al. 2001). Until now, no significant study of genetic diversity in V. mungo using DNA markers have been reported (Sivaprakash et al. 2004). The systematic collection of black gram has displayed inadequate variability for biotic and abiotic desirable genes. It is possible that genes for high productivity could have been lost due to the overriding role of natural selection (Roopalakshmi et al. 2003). The creation of novel variation through hybridization is difficult due to the prevalence of self pollination in V. mungo (Deepalakshmi and Anandakumar 2004) which lowers seed productivity, protein content, and other polygenic traits. Among the D. Arulbalachandran ( ) E-mail: [email protected] Institutional website: http://annamalaiuniversity.ac.in/genesis.htm
The Korean Soci
Data Loading...
