Genome characterization of brugmansia latent virus, a novel tobamovirus
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ANNOTATED SEQUENCE RECORD
Genome characterization of brugmansia latent virus, a novel tobamovirus Alison S. Scott‑Brown1 · Tom D’Elia2 · Dion S. Devey1 · Joseph E. Funderburk3 · Scott Adkins4 Received: 6 May 2020 / Accepted: 30 May 2020 © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020
Abstract A novel tobamovirus, brugmansia latent virus (BrLV), was discovered during a study of brugmansia (Brugmansia spp.) in the living collections held at the Royal Botanic Gardens, Kew. Here, we report the complete genome sequence of BrLV, which is 6,397 nucleotides long and contains the four open reading frames (RNA-dependent RNA polymerase, methyltransferase/ helicase, movement, and coat proteins) typical of tobamoviruses. The complete genome sequence of BrLV shares 69.7% nucleotide sequence identity with brugmansia mild mottle virus (BrMMV) and 66.7 to 68.7% identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the complete genome nucleotide sequence and the deduced amino acid sequences of the four tobamovirus proteins place BrLV in a subcluster with BrMMV within the Solanaceae-infecting tobamovirus subgroup as a new species. The solanaceous ornamental brugmansia (Brugmansia spp.) produces attractive, trumpet-shaped flowers leading to its common name of angel’s trumpet [3]. Prior research has shown that Colombian datura virus (CDV), a potyvirus, is frequently detected in brugmansia and related solanaceous plants worldwide [e.g., 5, 8]. Study of CDV genetic diversity and selection pressure estimates imply a timeline of CDV emergence that coincides with anthropogenic collection and dissemination of brugmansia [4]. To further explore this concept with CDV, brugmansia (Brugmansia spp.) accessions in the living collections of the Royal Botanic Gardens, Kew, with no obvious viral symptoms were examined for the presence of CDV, and concurrently tested for tobamoviruses, Handling Editor: Stephen John Wylie. * Alison S. Scott‑Brown a.scott‑[email protected] * Scott Adkins [email protected] 1
Royal Botanic Gardens Kew, Richmond, Surrey TW9 3AB, UK
2
Biology Department, Indian River State College, 3209 Virginia Avenue, Fort Pierce, FL 39481, USA
3
University of Florida, North Florida Research and Education Center, 155 Research Road, Quincy, FL 32351, USA
4
USDA, ARS, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA
by reverse transcription polymerase chain reaction. Total RNA extracts (RNeasy Plant Mini Kit, QIAGEN, Valencia, CA) from brugmansia leaves were used as template with the previously described CDV primers CDVv/CDVvc [4] and a mixture of previously described degenerate tobamovirus primers, SolACPv/SolACPvc and SolBCPv/SolBCPvc [4]. As in prior studies, CDV was widespread. Furthermore, a tobamovirus was detected in two independent brugmansia accessions (B. suaveolens collected in 1929; B. candida ‘Grand Marnier’ collected in 1996), bot
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