Genome Mining Reveals the Biosynthetic Pathways of Polyhydroxyalkanoate and Ectoines of the Halophilic Strain Salinivibr
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ORIGINAL ARTICLE
Genome Mining Reveals the Biosynthetic Pathways of Polyhydroxyalkanoate and Ectoines of the Halophilic Strain Salinivibrio proteolyticus M318 Isolated from Fermented Shrimp Paste Doan Van Thuoc 1 Kumar Sudesh 3
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& Tran Thi Loan & Trieu Anh Trung & Nguyen Van Quyen & Quach Ngoc Tung & Phi Quyet Tien &
Received: 14 May 2020 / Accepted: 3 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Salinivibrio proteolyticus M318, a halophilic bacterium isolated from fermented shrimp paste, is able to produce polyhydroxyalkanoate (PHA) from different carbon sources. In this study, we report the whole-genome sequence of strain M138, which comprises 2 separated chromosomes and 2 plasmids, and the complete genome contains 3,605,935 bp with an average GC content of 49.9%. The genome of strain M318 contains 3341 genes, 98 tRNA genes, and 28 rRNA genes. The 16S rRNA gene sequence and average nucleotide identity analysis associated with morphological and biochemical tests showed that this strain has high homology to the reference strain Salinivibrio proteolyticus DSM 8285. The genes encoding key enzymes for PHA and ectoine synthesis were identified from the bacterial genome. In addition, the TeaABC transporter responsible for ectoine uptake from the environment and the operon doeABXCD responsible for the degradation of ectoine were also detected. Strain M318 was able to produce poly(3-hydroxybutyrate) [P(3HB)] from different carbon sources such as glycerol, maltose, glucose, fructose, and starch. The ability to produce ectoines at different NaCl concentrations was investigated. High ectoine content of 26.2% of cell dry weight was obtained by this strain at 18% NaCl. This report provides genetic information regarding adaptive mechanisms of strain M318 to stress conditions, as well as new knowledge to facilitate the application of this strain as a bacterial cell factory for the production of PHA and ectoine. Keywords Complete genome sequence . Ectoine . Polyhydroxyalkanoate . Salinivibrio . Shrimp paste
Introduction Shrimp paste is a traditional product produced in Southeast Asian countries by natural fermentation of whole or ground Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10126-020-09986-z) contains supplementary material, which is available to authorized users. * Doan Van Thuoc [email protected] 1
Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam
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Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam
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School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
shrimp (Acetes shrimp) mixed with salt (10–15%) and exposed to sunlight for at least a month before utilization (Anh et al. 2015). A high number of halophilic and halotolerant bacteria were observed in shrimp paste (Kapi) during the first 30 days of fermentation (Pongsetkul et al. 2017). In order to live under th
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