Pan-genome analysis of Paenibacillus polymyxa strains reveals the mechanism of plant growth promotion and biocontrol
- PDF / 4,158,071 Bytes
- 20 Pages / 547.087 x 737.008 pts Page_size
- 42 Downloads / 193 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL PAPER
Pan-genome analysis of Paenibacillus polymyxa strains reveals the mechanism of plant growth promotion and biocontrol Liangliang Zhou . Ting Zhang . Shan Tang . Xueqin Fu . Shuijing Yu
Received: 22 May 2020 / Accepted: 4 August 2020 Ó Springer Nature Switzerland AG 2020
Abstract Rapid development of gene sequencing technologies has led to an exponential increase in microbial sequencing data. Genome research of a single organism does not capture the changes in the characteristics of genetic information within a species. Pan-genome analysis gives us a broader perspective to study the complete genetic information of a species. Paenibacillus polymyxa is a Gram-positive bacterium and an important plant growth-promoting rhizobacterium with the ability to produce multiple antibiotics, such as fusaricidin, lantibiotic, paenilan, and polymyxin. Our study explores the pan-genome of 14 representative P. polymyxa strains isolated from around the world. Heap’s law model and curve fitting
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01461-y) contains supplementary material, which is available to authorized users. L. Zhou S. Tang S. Yu (&) Faculty of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, People’s Republic of China e-mail: [email protected] T. Zhang College of Bioscience and Engineering, Jiangxi Agricultural university, Nanchang 330045, Jiangxi, People’s Republic of China X. Fu College of Life Science, Jiangxi Normal University, Nanchang 330022, Jiangxi, People’s Republic of China
confirmed an open pan-genome of P. polymyxa. The phylogenetic and collinearity analyses reflected that the evolutionary classification of P. polymyxa strains are not associated with geographical area and ecological niches. Few genes related to phytohormone synthesis and phosphate solubilization were conserved; however, the nif cluster gene associated with nitrogen fixation exists only in some strains. This finding is indicative of nitrogen fixing ability is not stable in P. polymyxa. Analysis of antibiotic gene clusters in P. polymyxa revealed the presence of these genes in both core and accessory genomes. This observation indicates that the difference in living environment led to loss of ability to synthesize antibiotics in some strains. The current pan-genomic analysis of P. polymyxa will help us understand the mechanisms of biological control and plant growth promotion. It will also promote the use of P. polymyxa in agriculture. Keywords Pan-genome Antibiotics Secondary metabolite Biocontrol Plant growth-promoting rhizobacteria
Introduction The advent of next generation sequencing (NGS) technology has provided a deeper understanding of
123
Antonie van Leeuwenhoek
basic cellular physiology, metagenome diversity, and microbial genetic libraries. It helped microbiologists discover significant differences in the bacterial genomes. The gen
Data Loading...
