Genome-wide identification and functional analysis of the TIFY gene family in the response to multiple stresses in Brass
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RESEARCH ARTICLE
Open Access
Genome-wide identification and functional analysis of the TIFY gene family in the response to multiple stresses in Brassica napus L. Xin He1,2,3, Yu Kang1, Wenqian Li1, Wei Liu1, Pan Xie1, Li Liao1, Luyao Huang1, Min Yao1, Lunwen Qian1, Zhongsong Liu2,3, Chunyun Guan2,3, Mei Guan2,3* and Wei Hua1,4*
Abstract Background: TIFY is a plant-specific protein family with a diversity of functions in plant development and responses to stress and hormones, which contains JASMONATE ZIM-domain (JAZ), TIFY, PPD and ZML subfamilies. Despite extensive studies of TIFY family in many other species, TIFY has not yet been characterized in Brassica napus. Results: In this study, we identified 77, 36 and 39 TIFY family genes in the genome of B. napus, B. rapa and B. oleracea, respectively. Results of the phylogenetic analysis indicated the 170 TIFY proteins from Arabidopsis, B. napus, B. rapa and B. oleracea could be divided into 11 groups: seven JAZ groups, one PPD group, one TIFY group, and two ZIM/ZML groups. The molecular evolutionary analysis showed that TIFY genes were conserved in Brassicaceae species. Gene expression profiling and qRT-PCR revealed that different groups of BnaTIFY members have distinct spatiotemporal expression patterns in normal conditions or following treatment with different abiotic/biotic stresses and hormones. The BnaJAZ subfamily genes were predominantly expressed in roots and up-regulated by NaCl, PEG, freezing, methyl jasmonate (MeJA), salicylic acid (SA) and Sclerotinia sclerotiorum in leaves, suggesting that they have a vital role in hormone signaling to regulate multiple stress tolerance in B. napus. Conclusions: The extensive annotation and expression analysis of the BnaTIFY genes contributes to our understanding of the functions of these genes in multiple stress responses and phytohormone crosstalk in B. napus. Keywords: Brassica napus, TIFY, Abiotic stresses, Freezing, Sclerotinia sclerotiorum, Hormone, MeJA
Background Plants often encounter various abiotic and biotic stresses during their life cycles, such as salinity, dehydration, extreme temperature and infection by pathogens. Plant hormones, such as abscisic acid (ABA), jasmonic acid (JA), ethylene (ET) and salicylic acid (SA) act as key defense signals in the * Correspondence: [email protected]; [email protected] 2 Oil Crops Research, Hunan Agricultural University, Changsha 410128, Hunan, China 1 Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, Changsha 410128, Hunan, China Full list of author information is available at the end of the article
regulation of various abiotic and biotic stress responses [1– 4]. There are many types of stress-related transcriptional factors and transcriptional regulators that function as important components in the crosstalk between hormone signals to regulate tolerance to stress in plants [5, 6]. TIFY is a plant-specific protein family with a diversity of functions in plant development and responses to stress and h
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