Genomic identification and expression analysis of the BBX transcription factor gene family in Petunia hybrida
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ORIGINAL ARTICLE
Genomic identification and expression analysis of the BBX transcription factor gene family in Petunia hybrida Shiyun Wen1 · Yue Zhang2,3 · Ying Deng4 · Guoju Chen4 · Yixun Yu1 · Qian Wei1 Received: 27 April 2020 / Accepted: 19 July 2020 © Springer Nature B.V. 2020
Abstract The B-box proteins (BBXs) are a class of zinc finger transcription factors containing one or two B-BOX domains that play important roles in plant growth, development and stress response. The petunia (Petunia hybrida) is a model ornamental plant, and its draft genome has been published. However, no systematic study of the BBX gene family in Petunia has been reported. In this study, a total of 28 BBX members from the Petunia genome were identified. We performed analyses of their phylogenetic relationships, structures, conserved motifs, promoter regions, and expression patterns. Based on the phylogenetic relationship, the PhBBXs were divided into six groups. Analysis of the gene structures and conserved motifs further confirmed the closer relationships in each group. Based on the RNA-seq data, the transcript abundance of PhBBXs in different tissues were divided into two major groups. The analysis of cis-elements showed that many stress responsive elements appeared in the promoter region of most PhBBX genes. The stress response patterns of PhBBXs were detected under drought, salinity, cold and heat treatments. Based on the RNA-seq data, we found that 3 genes responded to drought, 8 genes responded to salt, 18 genes responded to cold, and 15 genes responded to heat. In conclusion, this study may facilitate further functional studies of BBXs in Petunia. Keywords Petunia hybrida · BBX gene family · Gene expression · Abiotic stress
Introduction
Shiyun Wen and Yue Zhang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05678-y) contains supplementary material, which is available to authorized users. * Qian Wei [email protected] 1
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
2
Non‑timber Forestry Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, China
3
Key Laboratory of Non-timber Forest Germplasm Enhancement & Utilization of National Forestry and Grassland Administration, Zhengzhou 450003, China
4
College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Transcription factors (TFs) are a class of proteins that play important roles in plant development and stress response via regulating the transcription of target genes. This regulation is achieved through specific interaction between the DNA binding domain of the TFs and the promoters of the target genes. Beyond the DNA binding domain, TFs are also contained transcription activation domain, oligomerization site and nuclear localization signal [1]. These do
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