Glypican-3 electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids peroxidase-like catalytic silver de
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ORIGINAL PAPER
Glypican-3 electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids peroxidase-like catalytic silver deposition Zhide Zhou 1 & Le Zhao 1 & Wenzhan Li 1 & Min Chen 1 & Huafu Feng 1 & Xiaohang Shi 1 & Jintao Liang 1 & Guiyin Li 1 Received: 28 October 2019 / Accepted: 19 April 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A Glypican-3 (GPC3) electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids (HGNs) peroxidase-like catalytic silver deposition and GPC3 aptamer has been constructed for the determination of GPC3. The HGNs were prepared by an one-step reduction method. Fourier transform infrared spectroscopy (FT-IR), ultraviolet spectroscopy (UV-vis), and transmission electron microscopy (TEM) were used to study the structure and morphological characteristics of the HGNs. The GPC3 electrochemical aptamer nanobiosensor was constructed using HGNs-aptamer (HGNs-Apt) as the signal probe and GPC3 aptamer as the capture probe. With the help of the catalytic action of peroxidase-like properties of HGNs, H2O2 reduces the silver (Ag) ions in solution to metallic Ag, which deposit on the surface of the electrode. The amount of deposited Ag, which was derived from the amount of GPC3, was quantified by differential pulse voltammetry (DPV). Under optimal conditions, the current response of Ag had a good positive correlation with the GPC3 concentration in the range 10.0–100.0 μg mL−1 with a correlation coefficient of 0.9958. The detection limit was 3.16 μg mL−1 at a signal-to-noise ratio of 3, and the sensitivity was calculated to be 0.807 μA μM−1 cm−2. The method is validated by analyzing spiked human serum samples with good recovery ranging from 101 to 122%. In addition, the GPC3 electrochemical aptamer nanobiosensor has acceptable selectivity, stability, and reproducibility. Keywords Electrochemical nanobiosensor . Glypican-3 . Hemin/graphene nanohybrids . Peroxidase-like catalytic silver deposition . Aptamer
Introduction Hepatocellular carcinoma (HCC), also known as liver cancer, is one of the most common malignant tumors in the world; China has the highest incidence of liver cancer in the world [1, 2]. Glypican-3 (GPC3), a membrane-bound heparin sulfate
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04284-w) contains supplementary material, which is available to authorized users. * Jintao Liang [email protected] * Guiyin Li [email protected] 1
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, Guangxi, China
proteoglycan, is highly expressed in liver cancer tissues but negative in normal liver tissue or benign hepatocellular nodules [3, 4]. It can be released into the blood as a marker of liver cancer and may be applicable for the screening and diagnosis of liver cancer [5]. At present, enzyme-linked immunosorbent assay (ELISA), fluorescence immunoassay, electrochemical immunoassay, and chemiluminescent immunoassay are commonly used to det
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