Growth of Arabidopsis thaliana in rhizobox culture system evaluated through the lens of root microbiome
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Growth of Arabidopsis thaliana in rhizobox culture system evaluated through the lens of root microbiome Anne Mercier & Laure Mignerot & Nils Hennion & Kévin Gravouil & Benoît Porcheron & Mickaël Durand & Laurence Maurousset & Yann Héchard & Joanne Bertaux & Thierry Ferreira & Béatrice Lauga & Rémi Lemoine & Nathalie Pourtau
Received: 12 December 2019 / Accepted: 26 July 2020 # Springer Nature Switzerland AG 2020
Abstract Aims The present study provides an insight on physiological parameters and root-associated microbiome of two complementary Arabidopsis thaliana culture systems suitable for physiological studies. Methods A. thaliana plants were grown in rhizobox and in classic pot culture. An analysis of plant growth
parameters along with the characterization of the bacterial communities of the compost and the rhizosphere, rhizoplane and endosphere compartments were performed. Results A. thaliana plants grown in rhizobox exhibited a plant habitus similar to those grown in pots during the first month, but with a delayed leaf initiation and slower rate of growth over the experiment. No nutrient
Anne Mercier, Laure Mignerot and Nils Hennion contributed equally to this work. Responsible Editor: Luz E. de-Bashan. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04650-w) contains supplementary material, which is available to authorized users. A. Mercier : Y. Héchard Team Water Microbiology, Ecology and Biology of Interactions, UMR 7267, CNRS Université de Poitiers, 86073 Poitiers, France A. Mercier : K. Gravouil : T. Ferreira Thanaplast, Ecology and Biology of Interactions, UMR 7267, CNRS Université de Poitiers, 86073 Poitiers, France L. Mignerot : N. Hennion : B. Porcheron : M. Durand : L. Maurousset : R. Lemoine : N. Pourtau (*) Team Sugar and Exchanges Plant – Environment, Ecology and Biology of Interactions, UMR 7267, CNRS Université de Poitiers, 86073 Poitiers, France e-mail: [email protected] J. Bertaux Team Ecology, Evolution, Symbiosis, Ecology and Biology of Interactions, UMR 7267, CNRS Université de Poitiers, 86073 Poitiers, France
B. Lauga E2S UPPA, CNRS, IPREM, Institut des Sciences analytiques et de physico-chimie pour l’environnement et les matériaux, Université de Pau & Pays de l’Adour, 64000 Pau, France
Present Address: L. Mignerot Algal Genetics Group, UMR 8227 Integrative Biology of Marine Models, Station biologique de Roscoff, CS 90074, 29688 Roscoff, France Present Address: M. Durand Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559, Saclay Plant Sciences, INRA-AgroParisTech, Route de Saint-Cyr, 78026 Versailles, France
Plant Soil
deficiency symptoms were observed in these plants. The rhizobox design permits the migration of bacteria from compost to plant roots and allowed root-bacteria interactions to be established as in traditional pot culture. Some differences into the composition of the rhizobiome were highlighted between the two culture systems. Nevertheless, key bacterial taxa that help to ensur
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