Multigeneration Effects of Plasma Irradiation to Seeds of Arabidopsis Thaliana and Zinnia on Their Growth
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Multigeneration Effects of Plasma Irradiation to Seeds of Arabidopsis Thaliana and Zinnia on Their Growth Thapanut Sarinont1, Takaaki Amano1, Kazunori Koga1, Masaharu Shiratani1 and Nobuya Hayashi2 1
Graduate School of Information Science and Electrical Engineering, Kyushu University 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan 2 Interdisciplinary Graduate School of Engineering Science, Kyushu University 6-1 Kasuga-kouen, Fukuoka, 816-8580, Japan ABSTRACT We have studied multigeneration effects of plasma irradiation to seeds of Arabidopsis thaliana (L.) and Zinnia peruviana (L.) on their growth using a scalable DBD device. Atmospheric plasma irradiation enhances growth of these plants in multi-generations. For Arabidopsis thaliana (L.) in the third generation, the leaf area is 2 times larger than that without plasma irradiation and the stem length is 1.5 times longer than that without plasma. For Zinnia peruviana (L.) in the second generation, the stem length is 2 times longer than that without plasma. INTRODUCTION Recently, a wide variety of atmospheric pressure non-thermal plasma sources have been developed for medical and agricultural applications, since they can provide high density reactive species to living things without burning tissue [1-9]. Some plasma sources have been applied to inactivation of microorganisms on crops and foods, to improvement of seed germination, seed yield, plant growth, and to control of cell activities [7-9]. We have shown that atmospheric pressure air plasma irradiation to seeds can induce cell proliferation and continuous growth enhancement of several plants for weeks after their germination [6, 7]. The growth enhancement is attributed not to an increase of each cell volume but to an increase in the number of cells. Plasma irradiation may modify gene and gene expression of plants. Here we report effects of plasma irradiation on Arabidopsis thaliana and Zinnia reproduction in multigeneration test. Arabidopsis is as an attractive eukaryotic model for biology researches [10, 11], because all DNA sequences of Arabidopsis have been analyzed. Zinnia is a summer annual flower that is gaining rapid popularity for its variety of colorful blooms and provides a crucial step for developing in biofuel production species [12, 13]. EXPERIMENTAL DETAILS Experiments were carried out using a scalable DBD device as shown in figure 1 (a). The device consisted of 20 electrodes of a stainless rod of 1 mm in outer diameter and 60 mm in length covered with a ceramic tube of 2 mm in outer diameter. The electrodes were arranged parallel with each other at a distance of 0.2 mm. The discharge voltage and current were 9.2 kV and 0.2 A, respectively.
Arabidopsis thaliana (L.) and Zinnia Peruviana (L.) were used in this experiment. In order to shorten dormancy time of Arabidopsis thaliana, their seeds were sink into the DI water and were kept at 4 oC for 4 days. 8 seeds of Arabidopsis thaliana (L.) and Zinnia Peruviana (L.) were set below the electrodes as in figure 1 (b). Plasma was irradiated to these see
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