Growth, Physiological and Biochemical Traits of Sage under the Exogenous Stimulating and Stress Factors
- PDF / 562,224 Bytes
- 12 Pages / 612 x 792 pts (letter) Page_size
- 80 Downloads / 206 Views
ESEARCH PAPERS
Growth, Physiological and Biochemical Traits of Sage under the Exogenous Stimulating and Stress Factors A. Karimia, F. Rahmania, *, A. Ghasemi Pirbaloutib, c, **, and M. Mohamadid aDepartment
of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Center for Medicinal Plants, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran c Medicinal Plants Program, Collage of Natural Sciences, University of Massachusetts, Amherst, MA, USA dAgricultural and Natural Resources Research Center of Chaharmahal va Bakhtiari Province, Shahrekord, Iran *e-mail: [email protected] **e-mail: [email protected] bResearch
Received July 29, 2019; revised December 26, 2019; accepted February 12, 2020
Abstract—Sage (Salvia officinalis L.) belonging to the Lamiaceae family, as the main herb in the world is cultivated in many countries for extraction of essential oil from the leaves. One of the most important uses of the plant is to use its leaves as raw material in pharmaceutical and cosmetics industries. Interaction effects of exogenous jasmonic acid (JA) at different concentrations (0.00, 400, and 600 μM) and the applied of arbuscular mycorrhizal fungi (AMF) in soil under different moisture conditions (control or non-stress, slightdrought, and mild drought stress) on growth, and morphophysiological characteristics of sage and the quantity and quality yield of essential oil from the sage leaves were investigated. The constituents in the essential oils from the sage leaves were analyzed by GC-FID and GC/MS. Results indicated that the inoculation with AMF meaningfully increased the growth parameters sage, while, the foliar spray of JA improved only physiological traits such as soluble sugar, proline, and anthocyanin in the leaves. Experimental treatments individually increased the concentrations of 1,8-cineole in the essential oils. The interaction effects including AMF × JA, AMF × irrigation, and JA × irrigation significantly affected the essential oil yield and the morphological characteristic of sage. The highest value of the essential oil yield was obtained from AMF-inoculation × mild drought × JA at 400 μM. Finally, the exogenous application of JA along inoculation with AMF changed some characteristics of the sage plant under drought stress condition. Indeed, AMF-inoculation and the foliar spraying of JA had synergistic effects on the amounts secondary metabolites of sage especially, 1,8-cineole. In conclusion, we found that the exogenous JA along the inoculation of arbuscular mycorrhizal improves growth characteristics and quality of the essential oil of sage and enhances water deficit tolerance. Keywords: Salvia officinalis, drought stress, arbuscular mycorrhizal fungi, proline, secondary metabolites, jasmonic acid DOI: 10.1134/S1021443720050076
INTRODUCTION Sage (Salvia officinalis L.) belonging to the family Lamiaceae is an important aromatic and medicinal plant. This herb is cultivated in various countries to be used as raw material in food, pharmaceutical, and perfumery industries [1]. In ethno-
Data Loading...
