Heterologous mesenchymal stem cells successfully treat femoral pseudarthrosis in rats
- PDF / 564,572 Bytes
- 10 Pages / 595.28 x 793.7 pts Page_size
- 20 Downloads / 162 Views
RESEARCH
Open Access
Heterologous mesenchymal stem cells successfully treat femoral pseudarthrosis in rats Manoel Luiz Ferreira1,6*, Paulo Cesar Silva2, Lucas Henrique Alvarez Silva2, Danielle Cabral Bonfim3, Lucas Cristo Conilho Macedo Müller2, Christiano Costa Espósito2,4 and Alberto Schanaider1,2,4,5
Abstract Background: This study evaluated the effectiveness of treating pseudarthrosis in rats by using bone marrow cell suspensions or cultures of bone marrow mesenchymal stromal cells Methods: Thirty-eight specific pathogen-free (SPF) animals were randomly assigned to four groups: Group 1, Control, without surgical intervention; Group 2 (Placebo), experimental model of femoral pseudarthrosis treated only with saline solution; Group 3, experimental model of femoral pseudarthrosis treated with heterologous bone marrow cells suspension; Group 4, experimental model of femoral pseudarthrosis treated with cultures of heterologous mesenchymal stromal cells from bone marrow. When pseudarthrosis was confirmed by simple radiological studies, digital radiography and histopathology after a 120-day postoperative period, Groups 2, 3 and 4 were treated as above. At 30, 60 and 90 days after the treatment, all animals were evaluated by simple radiological studies, and at the end of the experiment, the animals were assessed by computed axial tomography and anatomopathological and histomorphometric examinations. Results: Injected cells were detected in the areas affected by pseudarthrosis using scintigraphy within the first 24 hours after their administration. After 60 days, the animals of Group 3 showed callus formation while the animals of Group 4 presented periosteal reaction and had some consolidated areas. In contrast, Group 2 showed a predominance of fibro-osteoid tissue. After 90 days, bone consolidation and remodeling was observed in all animals from Group 3 whereas animals from Group 4 exhibited partial consolidation and those ones from Group 2 persisted with pseudarthrosis. Conclusion: The treatment with heterologous bone marrow cells suspension proved to be effective in the treatment of pseudarthrosis whereas cultures of heterologous bone marrow mesenchymal stromal cells did not show the same potential to aid bone healing. Keywords: Rats, Pseudarthrosis, Osteotomies, Animal disease models, Bone marrow, Stromal cells
Background The term pseudarthrosis defines the nonunion of a fracture when the bone repair process has ceased for any reason. The lack of union of fractured bone surfaces in such circumstances results from the presence of fibrocartilage or fibrous tissues between the bone extremities [1]. Other factors that contribute to the persistence of this problem are the instability of the fracture, the lack of adequate blood supply and the presence of infection. * Correspondence: [email protected] 1 Post-graduate Program in Surgical Sciences, Department of Surgery, School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Full list of author information is available at the end of the artic
Data Loading...
