Heterotrophic Microalgae Cultivation Using Jellyfish Protein Extract to Produce Bioactive Peptides
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ORIGINAL PAPER
Heterotrophic Microalgae Cultivation Using Jellyfish Protein Extract to Produce Bioactive Peptides Nobuhiro Aburai1 · Junko Oe1 · Katsuya Abe1 Received: 22 February 2020 / Accepted: 25 September 2020 © Springer Nature B.V. 2020
Abstract Jellyfish are often seen as a nuisance, and while attempts have been made to use jellyfish waste as fertilizer and food, they have not proved fruitful. In this study, proteins extracted from jellyfish (Aurelia aurita) are investigated as a novel nutrient source in culture medium and tested on aerial microalgae under heterotrophic condition. Results demonstrated that Vischeria helvetica KGU-Y001 heterotrophically grew in the extract-containing culture medium in the presence of light. Decomposition of the extracted protein in the culture medium containing V. helvetica cells was verified both in the presence and absence of light. The inhibitory activity of angiotensin I-converting enzyme (ACE) was measured in the culture medium when in the presence of light. The hydrolysates in the medium were separated using a gel filtration column and a peptide fraction with approximately 400 Da, and high ACE inhibitory activity ( IC50 = 72 µg mL−1) was obtained. The results presented here will help to facilitate the development of culture systems with jellyfish waste as a resource for culture medium. Graphic Abstract
Keywords Jellyfish · Aerial microalga · Bioactive materials · Waste · Protease · Bioprocess
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12649-020-01261-5) contains supplementary material, which is available to authorized users. * Nobuhiro Aburai [email protected] 1
Statement of Novelty This research presents the development of a production system for useful materials from photosynthetic microorganisms using jellyfish waste as a resource in culture medium.
Department of Chemistry and Life Science, School of Advanced Engineering, Kogakuin University, 2665‑1 Nakano‑machi, Hachioji, Tokyo 192‑0015, Japan
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Introduction Jellyfish are evolutionarily interesting organisms [1]. The fishing industry is adversely affected by the annual mass appearance of jellyfish. Additionally, jellyfish accumulate in the tunnels of coastal power plants, which reduces water flow and sometimes causes plant shutdowns. Because of this, jellyfish are often referred to as “a nuisance” and “perpetual troublemakers” [2]. Although attempts have been made to use jellyfish waste as fertilizer and food, jellyfish biomass has proven to have little use to date. Another potential avenue for the industrial use of jellyfish is to obtain collagen from the mesoglea of rhizostomous jellyfish [3]. Recently, promising work found that a novel glycoprotein (a member of the mucin family) could be extracted from five species of jellyfish with high yields and that the glycoprotein collected was suitable for synthesizing tailor-made mucins [4]. Furthermore, unicellular aerial microalga Elliptochloris reniformis could be cu
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