Over expression of a Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease in transge
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Plant Biotechnology Reports (2020) 14:725–736 https://doi.org/10.1007/s11816-020-00648-z
ORIGINAL ARTICLE
Over expression of a Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease in transgenic Arabidopsis thaliana Zhifeng Wen1 · Jinhui Bai1 · Lin Wang1 · Liping Yao2 · Bilal Ahmad3 · Muhammad Hanif4 · Qingxi Chen1 Received: 24 June 2020 / Revised: 1 November 2020 / Accepted: 15 November 2020 / Published online: 25 November 2020 © Korean Society for Plant Biotechnology 2020
Abstract Strawberry (Fragaria × ananassa Duch.) is one of the most widely cultivated fruit crop. Anthracnose caused by Colletotrichum spp. is a devastating disease of strawberry, causing large-scale strawberry losses worldwide. Chitinases act as defence proteins and are crucial for plant response to pathogens. Here, we isolated a class V Chitinase gene (designed as FnCHIT2, GenBank accession number MN709779) from Chinese wild diploid strawberry Fragaria nilgerrensis Schlecht (F. nilgerrensis), a species that exhibits high tolerance to anthracnose. Gene expression analysis showed that FnCHIT2 expression was highly induced after Colletotrichum gloeosporiodes inoculation and salicylic acid (SA) treatment. Subcellular localization analysis revealed the presence of FnCHIT2 in the plasma membrane. Recombinant FnCHIT2 protein was successfully expressed in E. coli Rosetta (DE3). Furthermore, we transformed FnCHIT2 into Col-0 wild type A. thaliana to perform functional analysis and evaluated the functions of Colletotrichum higginsianum and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). FnCHIT2 overexpression in A. thaliana showed enhanced resistance to C. higginsianum and Pst DC3000. Enhanced disease resistance of FnCHIT2 transgenic plants to C. higginsianum was correlated with pathogenesis-related gene 1 (AtPR1) and plant defensin 1.2 (AtPDF1.2) gene expression levels. These results provide evidence that FnCHIT2 may play an important role in response to fungal pathogens in strawberry. Our study provides an important theoretical reference for future strawberry resistance breeding. Keyword Strawberry · Anthracnose · FnCHIT2 · Signal transduction pathways Abbreviations F. nilgerrensis Fragaria nilgerrensis Schlecht C. gloeosporiodes Colletotrichum gloesporioides SA Salicylic acid Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11816-020-00648-z) contains supplementary material, which is available to authorized users. * Zhifeng Wen [email protected] 1
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Fuzhou Institute of Agricultural Science, Fuzhou 350018, China
3
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A and F University, Yangling 712100, Shaanxi, China
4
Horticulture Research Institute, National Agricultural Research Centre, Islamabad 44000, Pakistan
JA Jasmonic acid A. thaliana Arabidopsis thaliana Pst DC3000 Pseudomonas syringae Pv.
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