Highly polymorphic microsatellite markers in two species, the invasive shore crabs Hemigrapsus sanguineus and Hemigrapsu
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MICROSATELLITE LETTERS
Highly polymorphic microsatellite markers in two species, the invasive shore crabs Hemigrapsus sanguineus and Hemigrapsus takanoi (Decapoda, Varunidae) Ce´line Poux • Moaˆna Gothland • Anne-Catherine Holl Nicolas Spilmont • Jean-Franc¸ois Arnaud
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Received: 6 January 2015 / Accepted: 7 January 2015 Ó Springer Science+Business Media Dordrecht 2015
Abstract Varunid crabs Hemigrapsus sanguineus and Hemigrapsus takanoi are species native from intertidal coastal areas in North-western Pacific and have become invasive along the Atlantic European coasts. To gain insights into population genetic features, we developed and characterized 16 (H. sanguineus) and 11 (H. takanoi) novel polymorphic microsatellite loci from next-generation sequencing. The number of alleles ranged from 2 to 20 for H. sanguineus, and from 8 to 21 for H. takanoi. Expected heterozygosity ranged from 0.470 to 0.947 and from 0.313 to 0.781, with mean multilocus FIS estimates suggesting rapid turnover of populations. Overall, these microsatellite markers showed very high levels of polymorphism that will facilitate population genetic studies devoted to track down the most likely sources of introduction. Keywords Hemigrapsus Biological invasion Intertidal coastal areas Microsatellites
Electronic supplementary material The online version of this article (doi:10.1007/s12686-015-0426-6) contains supplementary material, which is available to authorized users. C. Poux A.-C. Holl J.-F. Arnaud (&) Unite´ E´volution, E´cologie, Pale´ontologie, UMR CNRS 8198, Universite´ Lille 1 – Sciences et Technologies, 59655 Villeneuve d’Ascq Cedex, France e-mail: [email protected] M. Gothland N. Spilmont Laboratoire d’Oce´anologie et de Ge´osciences, UMR CNRS 8187, Universite´ Lille 1 – Sciences et Technologies, 62930 Wimereux, France N. Spilmont Environmental Futures Research Institute, Grifith University, Gold Coast, QLD 4222, Australia
Biological invasions are often closely linked to anthropogenic activities. Maritime trade promotes recurrent humaninduced migration from native ranges, disrupting the dynamic of natural coastal ecosystems. Among maritime intertidal alien species identified in Europe, two Asian shore crab species Hemigrapsus sanguineus and Hemigrapsus takanoi (Asakura and Watanabe, 2005) have been reported along the Atlantic coast, from southern France to North Sea in Germany. Both species are native from the North-western Pacific. To track down invasive spread and the most likely sources of introduction, we isolated set of microsatellites markers for both species. Total genomic DNA from H. sanguineus and H. takanoi was extracted using the NucleoSpinÒ Tissue kit (Macherey–Nagel, Duren, Germany) following protocols outlined in the manufacturer’s handbook and sent to GenoScreen, Lille, France (www.genoscreen.fr). DNA libraries were constructed by coupling multiplex microsatellite enrichment and next generation sequencing as described in Malausa et al. (2011). 1 lg of DNA was used to develop microsatellit
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