Human Cell Culture Volume IV: Primary Hematopoietic Cells
The daily production of hundreds of billions of blood cells through the process of hematopoiesis is a remarkable feat of human physiology. Transport of oxygen to tissues, blood clotting, antibody- and cellular-mediated immunity, bone remodeling, and a hos
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Human Cell Culture Volume 4
Human Cell Culture Volume IV: Primary Hematopoietic Cells edited by
Manfred R. Koller Oncosis, San Diego, CA, U.S.A.
BernhardO.Palsson University of California, Department of Bioengineering, La Jolla, CA, U. S.A.
and
John R.W. Masters University College London, Institute of Urology, London, U.K.
KLUWER ACADEMIC PUBLISHERS
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eBook ISBN: Print ISBN:
0-306-46886-7 0-792-35821-X
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Table of Contents Chapter 1: Hematopoietic stem and progenitor cells Manfred R. Koller, Ph.D. Oncosis Bernhard 0. Palsson, Ph.D. University of California - San Diego
1
Chapter 2: In vitro T-lymphopoiesis Michael Rosenzweig, Ph.D. New England Regional Primate Center David T. Scadden, M.D. Massachusetts General Hospital
31
Chapter 3: T-lymphocytes: Mature polyclonal and antigen-specific cell expansion Bruce L. Levine, Ph.D. Naval Medical Research Institute Katia Schlienger, M.D., Ph.D. Naval Medical Research Institute Carl H. June, M.D. Naval Medical Research Institute
45
Chapter 4: The culture, characterization, and triggering of B lymphocytes Gerrard Teoh, M.D. Dana-Farber Cancer Institute Kenneth C. Anderson, M.D. Dana-Farber Cancer Institute
101
Chapter 5: Monocytes and macrophages Ivan N. Rich, Ph.D. Richland Memorial Hospital
125
Chapter 6: Isolation and cultivation of osteoclasts and osteoclast-like cells Philip A. Osdoby, Ph.D. Washington University Fred Anderson. Washington University William Maloney, M.D. Washington University Medical Center Patricia Collin-Osdoby, Ph.D. Washington University
147
Chapter 7: Isolation and culture of human dendritic cells Michael A. Morse, M.D. Duke University H. Kim Lyerly, M.D. Duke University
171
Chapter 8: In vitro proliferation and differentiation of CD34+ cells to neutrophils James G. Bender, Ph.D. Nexell Therapeutics, Inc.
193
Chapter 9: Isolation and culture of eosinophils Helene F. Rosenberg, M.D. National Institutes of Health
219
Chapter 10: Isolation and culture of mast cells and basophils Peter Valent, M.D. University of Vienna
241
Chapter 11: Purification and culture of erythroid progenitor cells Chun-Hua Dai, M.D. VA Medical Center Amittha Wickrema, Ph.D. University of Illinois - Chicago Sanford B. Krantz, M.D. Vanderbilt University Medical School
259
Chapter 12: In vitro development of megakaryocytes and platelets Marcus 0. Muench. Ph.D. University of California - San Francisco Jae-Hung Shieh, Ph.D. New York Blood Center
287
Chapter 13: Perspectives. ethics, and clinical issues in the use of primary human cells Mary Pat Moyer. Ph.D. InCelI, Inc.
317
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