Human Cell Culture Volume VI: Embryonic Stem Cells
If you wish to grow or characterize embryonic stem cells or persuade them to differentiate into a particular cell type, then this book contains information that is vital to your success. The aim is to provide clear simple instructions and protocols for gr
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Embryonic Stem Cells
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HUMAN CELL CULTURE Volume VI: Embryonic Stem Cells
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Human Cell Culture Volume 6
The titles published in this series are listed at the end of this volume.
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Human Cell Culture Volume VI Embryonic Stem Cells edited by
John R. Masters University College London, London, UK
Bernhard O. Palsson University of California, San Diego, CA, USA and
James A. Thomson University of Wisconsin, Madison, WI, USA
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A C.I.P. Catalogue record for this book is available from the Library of Congress.
ISBN-13 978-1-4020-5982-7 (HB) ISBN-13 978-1-4020-5983-4 (e-book)
Published by Springer, P.O. Box 17, 3300 AA Dordrecht, The Netherlands. www.springer.com
Printed on acid-free paper
All Rights Reserved © 2007 Springer No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work.
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CONTENTS
Preface
vii
1. Defined Culture Media for Human Embryonic Stem Cells Tenneille Ludwig and James A. Thomson
1
2. Generation of Disease-specific Human Embryonic Stem Cell Lines Stephen Minger
17
3. Characterization and Differentiation of Human Embryonic Stem Cells Andrew L. Laslett, Adelia Lin, and Martin F. Pera
27
4. Genetic Modification of Human Embryonic Stem Cells Thomas P. Zwaka
41
5. Hematopoietic Differentiation Chantal Cerdan, Veronica Ramos-Mejia, and Mickie Bhatia
53
6. Neural Differentiation Zhi-Jian Zhang, Jason S. Meyer, and Su-Chun Zhang
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7. Germ Cell Differentiation Vanessa T. Angeles and Renee A. Reijo Pera
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8. Mesodermal Differentiation Nadav Sharon and Nissim Benvenisty
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v
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vi 9. Three-dimensional Culture of Human Embryonic Stem Cells Sharon Gerecht, Jason A. Burdick, Christopher Cannizzaro, and Gordana Vunjak-Novakovic
Contents 149
10. Extraembryonic Cell Differentiation Lyle Armstrong and Majlinda Lako
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11. Pancreatic Cell Differentiation Bettina Fishman, Hanna Segev, and Joseph Itskovitz-Eldor
189
12. Cardiomyocyte Differentiation Dinender K. Singla, Shreeya Jayaraman, Jianhua Zhang, and Timothy J. Kamp
211
13. Human Embryonal Carcinoma (EC) Cells: Complementary Tools for Embryonic Stem Cell Research Peter D. Tonge and Peter W. Andrews
235
14. Quality Control of Human Stem Cell Lines Glyn N. Stacey
255
Index
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PREFACE
The aim of this volume is to describe methods for culturing human embryonic stem cells and the culture conditions needed to direct these cells to differentiate into specialized cell types. Human embryonic stem cells are potentially capable of differentiation in
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