Human Embryonic Stem Cell Protocols
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renew
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M O L E C U L A R B I O L O G Y TM
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
Human Embryonic Stem Cell Protocols Second Edition Edited by
Kursad Turksen Ottawa Hospital Research Institute, Ottawa, Ontario, Canada
Editor Kursad Turksen Ottawa Hospital Research Institute (OHRI) Sprott Centre for Stem Cell Research 501 Smyth Road, Ottawa ON K1H 8L6 Canada [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-368-8 e-ISBN 978-1-60761-369-5 DOI 10.1007/978-1-60761-369-5 Library of Congress Control Number: 2009935009 # Humana Press, a part of Springer ScienceþBusiness Media, LLC 2006, 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer ScienceþBusiness Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper springer.com
Preface Embryonic stem (ES) cells, and more specifically human ES cells, remain promising tools for understanding questions of lineage commitment and for exploring potentialities for regenerative medicine applications. In this second edition, I have tried to bring together a number of new protocols that will extend the topics and reflect new developments since publication of the first edition. I would like to take this opportunity to thank all the contributors for very graciously providing their protocols for this volume. Without them and their willingness to share protocol details, this new volume would not have materialized. I would also like to thank Dr. John Walker, the Editor-in-Chief of the Methods in Molecular Biology series, for his continued support. Patrick Marton, the Editor of the Methods in Molecular Biology series at Springer, also deserves thanks for always being available to answer my questions, for patiently listening to my suggestions, and for supporting this volume during its maturation stages. Thanks also to David Casey for his invaluable help during the production stages of this volume. Kursad Turksen
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Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.
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Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)
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