Human papillomavirus detection using the Abbott RealTi m e high-risk HPV tests compared with conventional nested PCR cou
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RESEARCH
Open Access
Human papillomavirus detection using the Abbott RealTime high-risk HPV tests compared with conventional nested PCR coupled to high-throughput sequencing of amplification products in cervical smear specimens from a Gabonese female population Pamela Moussavou-Boundzanga1, Ismaël Hervé Koumakpayi2, Ingrid Labouba1, Eric M. Leroy1,3, Ernest Belembaogo2 and Nicolas Berthet1,4,5*
Abstract Background: Cervical cancer is the fourth most common malignancy in women worldwide. However, screening with human papillomavirus (HPV) molecular tests holds promise for reducing cervical cancer incidence and mortality in low- and middle-income countries. The performance of the Abbott RealTime High-Risk HPV test (AbRT) was evaluated in 83 cervical smear specimens and compared with a conventional nested PCR coupled to high-throughput sequencing (HTS) to identify the amplicons. Results: The AbRT assay detected at least one HPV genotype in 44.57% of women regardless of the grade of cervical abnormalities. Except for one case, good concordance was observed for the genotypes detected with the AbRT assay in the high-risk HPV category determined with HTS of the amplicon generated by conventional nested PCR. Conclusions: The AbRT test is an easy and reliable molecular tool and was as sensitive as conventional nested PCR in cervical smear specimens for detection HPVs associated with high-grade lesions. Moreover, sequencing amplicons using an HTS approach effectively identified the genotype of the hrHPV identified with the AbRT test. Keywords: Abbott RealTime high-risk HPV, Conventional PCR, Human papillomavirus, Screening, Cervical cancer
Background Infections with human papillomavirus (HPV) are the most common sexually transmitted infections worldwide [1]. Persistent cervical infection is a high risk factor for cervical cancer, which is the fourth most common * Correspondence: [email protected] 1 Centre International de Recherches Médicales de Franceville (CIRMF), Department of Zoonosis and Emerging Diseases, 769 Franceville, BP, Gabon 4 Centre National de Recherche Scientifique (CNRS), UMR3569, 25 rue du docteur Roux, 75724 Paris, France Full list of author information is available at the end of the article
malignancy in women worldwide. Moreover, there is a clear etiological link between the persistence of HPV infections in epithelial cells of the cervical mucosa, precancerous lesions (i.e., cervical intraepithelial neoplasia 3; CIN3) and cervical cancer [2]. Today, more than 200 HPV genotypes have been defined, among which 50 can infect the cervical epithelia. However, only 16 HPV types (16, 18, 31, 33, 34, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, 70) are classified as high risk (hrHPVs) and 14 (excluding 34 and 59) show strong evidence of a causal link to cervical cancer [3].
© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and r
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