Human Umbilical Cord Tissue-Derived Multipotent Mesenchymal Stromal Cells Exhibit Maximum Secretory Activity in the Pres
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Cell Technologies in Biology and Medicine, No. 2, August, 2020
Human Umbilical Cord Tissue-Derived Multipotent Mesenchymal Stromal Cells Exhibit Maximum Secretory Activity in the Presence of Umbilical Cord Blood Serum
Yu. A. Romanov1,3, V. V. Vtorushina2, T. N. Dugina3, A. Yu. Romanov2, N. V. Petrova3, and G. T. Sukhikh2 Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 84-88, June, 2020 Original article submitted February 20, 2020 Using multiplex analysis, we performed a comparative study of cytokine and growth factor production by human umbilical cord tissue-derived multipotent mesenchymal stromal cells (UC-MSC) cultured under standard conditions and in the presence of human umbilical cord blood serum (UCBS). It was found that the secretion of most studied molecules, including well-known inductors of regeneration HGF, G-CSF, GM-CSF, and VEGF by UCMSC considerably increased in the presence of 5% UCBS. The use of UCBS allows not only obtaining xenogenic-free cellular and cell-free therapeutic products, but also increasing the secretion of most biologically active molecules capable of stimulating repair processes. Key Words: multipotent mesenchymal stromal cells; umbilical cord; cord blood serum; cytokines; growth factors
Most pathological conditions are accompanied by degeneration of cellular components of organs and tissue systems. Spontaneous regeneration does not always lead to the desired result, which necessitates the development of various approaches to stimulation of this process, including cell therapy. One of the most promising sources of cells for regenerative medicine are tissues of postnatal origin, in particular, umbilical cord (UC) tissue as the source of multipotent mesenchymal stromal cells (MSC). The absence of ethical and biological problems, simple procedure of isolation, high proliferative and secretory activity of these cells in comparison with MSC from other available sources of postnatal origin [5,12,19] allow considering UC-MSC as the optimal tool for cell therapy of various diseases and pathological conditions [5,6,8,11,21]. National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; 2V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; 3CryoCenter Cord Blood Bank, Moscow, Russia. Address for correspondence: romanov@cryocenter. ru. Yu. A. Romanov 1
Due to their capacity to differentiate into cells of various organs and tissues, MSC can directly participate in the reparative regeneration by replacing damaged cells and replenishing tissue defects [13,21]. However, after systemic administration of MSC, only a few of them reach the target tissue; and even after local infusion, they usually survive for a short time [16], especially in cases of allogeneic transplantation [7]. Thus, the effects of MSC can be achieved due to other mechanisms, including paracrine regulation of various components of homeostasis [6,9,18,19]. In this regard, MSC-secre
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