Regenerative Potential of a Suspension and Spheroids of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord
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Cell Technologies in Biology and Medicine, No. 2, August, 2020
Regenerative Potential of a Suspension and Spheroids of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord on the Model of Myocardial Infarction in Rats M. E. Krasina1, N. V. Kosheleva1,2,3, T. V. Lipina1, M. Yu. Karganov2, Yu. S. Medvedeva2, M. A. Lebedeva2, I. M. Zurina2,3,4, and I. N. Saburina2,3 Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 89-97, June, 2020 Original article submitted July 2, 2019 Regenerative potential of multipotent mesenchymal stromal cells from the human umbilical cord (MMSC-UC) in the suspension and spheroid form was revealed during the progression of experimental small focal myocardial infarction in rats. In isoproterenol-induced myocardial infarction, foci of necrosis and inflammatory infiltrate and at later terms fibrosis foci were found mainly in the left ventricle of rat heart. In rats receiving MMSC-UC, destructive changes in the myocardium, fibrous scars, and inflammatory process were less pronounced. MMSC-UC also contributed to normalization of the morphofunctional parameters of the heart. Spheroids exhibited higher efficiency in comparison with cell suspension. Key Words: multipotent mesenchymal stromal cells; myocardial infarction; spheroids; isoproterenol In the absence of sufficient oxygen supply to the tissue in any part of the heart muscle, damage and necrotic death of cardiomyocytes occur due to various causes [11]. Then, the inflammatory response is activated, the pool of dead cardiomyocytes is not restored and these areas are replaced by a connective tissue scar [7], which impairs heart contractility and leads to heart failure [15]. Among the various methods of modeling myocardial infarction in experimental animals, pharmacological model of heart tissue damage using isoproterenol (1-(3,4-dioxyphenyl)-2-isopropylaminoethanol hydrochloride, a synthetic catecholamine, β1-adrenergic receptor agonist is often used. Isoproterenol induces ischemic damage to the myocardium, followed by neFaculty of Biology, M. V. Lomonosov Moscow State University; Research Institute of General Pathology and Pathophysiology; 3Russian Medical Academy of Continuous Professional Education, Mini stry of Health of the Russian Federation; 4Institute of Regenerative Medicine, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia. Address for correspondence: [email protected]. M. E. Krasina 1 2
crosis and inflammation leading to heart hypertrophy and formation of fibrous tissue between cardiomyocytes of the left ventricle (LV) [3]. Isoproterenolinduced diffuse foci of injury in the myocardium are most often observed in the LV subendocardium and subepicardium, heart apex, interventricular septum, and subendocardium of the right ventricle [5]. Thus, the pathological changes in the myocardium caused by isoproterenol are similar to those in myocardial infarction in humans. In regenerative medicine, multipotent mesenc
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