Hybrid of niosomes and bio-synthesized selenium nanoparticles as a novel approach in drug delivery for cancer treatment
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ORIGINAL ARTICLE
Hybrid of niosomes and bio‑synthesized selenium nanoparticles as a novel approach in drug delivery for cancer treatment Mahmoud Gharbavi1,2,3 · Behrooz Johari4 · Navid Mousazadeh4 · Bahareh Rahimi5 · Milad Parvinzad Leilan4 · Seyed Sadegh Eslami6 · Ali Sharafi3 Received: 5 June 2020 / Accepted: 1 August 2020 © Springer Nature B.V. 2020
Abstract The current study intends to investigate a novel drug delivery system (DDS) based on niosomes structure (NISM) and bovine serum albumin (BSA) which was formulated to BSA coated NISM (NISM-B). Also, selenium nanoparticles (SeNPs) have been prepared by BSA mediated biosynthesis. Finally, the NISM-B was hybridized with SeNPs and was formulated as NISM-B@SeNPs for drug delivery applications. Physicochemical properties of all samples were characterized by UV–Vis spectroscopy, FT-IR, DLS, FESEM, and EDX techniques. The cytotoxicity of all samples against A549 cell line was assessed by cell viability analysis and flow cytometry for apoptotic cells as well as RT-PCR for the expression of MDR-1, Bax, and Bcl-2 genes. Besides, in vivo biocompatibility was performed by L D50 assay to evaluate the acute toxicity. The proposed formulation has a regular spherical shape and approximately narrow size distribution with proper zeta-potential values; the proposed DDS revealed a good biocompatibility. The compound showed a significant cytotoxic effect against A549 cell line. Although the Bax/Bcl-2 expression ratio was significantly in NISM-B@SeNPs- treated cancer cells, the expression of MDR-1 was non-significantly lower in NISM-B@SeNPs-treated cancer cells. The obtained results suggest that the proposed DDS presents a promising approach for drug delivery, co-delivery and multifunctional biomedicine applications.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05704-z) contains supplementary material, which is available to authorized users. * Behrooz Johari [email protected]
3
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
* Ali Sharafi [email protected]
4
Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Navid Mousazadeh [email protected]
5
Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
Seyed Sadegh Eslami [email protected]
6
Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
1
Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran
Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
2
13
Vol.:(0123456789)
Molecular Biology Reports
Graphic abstract
Keywords Albumin · Biosynthesis · BSA · Drug delivery · Selenium nanoparticles · Niosomes Abbreviations BSA Bovine serum albumin NISM-B BSA coated NISM cDNA Compleme
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