Identification and analysis of new mycoviruses from melon powdery mildew
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ORIGINAL ARTICLE
Identification and analysis of new mycoviruses from melon powdery mildew Yushanjiang Maimaiti 1 & Lei Ding 2,3 & Min Chai 2 & Xiaoya Jing 2 & Du Yang 1 & Sheng Han 1 & Liuqing Sun 2 & Wei Chen 2 Received: 6 June 2019 / Accepted: 26 June 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract Melon powdery mildew is a devastating disease caused by Podosphaera xanthii. To identify and characterize mycoviruses of P. xanthii, total RNA of P. xanthi was extracted for constructing a library which was sequenced in a paired-end 2 × 75 bp mode of a NextSeq500 sequencer. Sequence analyses showed 87,607,242 clean reads, and 1076 unigenes were successfully matched with 151 mycovirus isolates within 71 mycovirus species. Among these isolates, 17 new isolates from four mycovirus species were identified. Their amino acid identity ranged from 32.8% to 100%. Phylogenetic relationships based on the RNA-dependent RNA polymerase protein of 37 isolates, including 20 retrieved from databases, showed a high divergence, which was confirmed by differences in genome structure and size. These results expand our understanding of the diversity of mycoviruses and will be useful for the development of potential biological control methods. Keywords Mycoviruses . Podosphaera xanthii . Genetic diversity . Biological control
Introduction Mycoviruses can be challenging to study because of imperceptible effect on the host and difficulty to detect (Nuss 2005; Adams et al. 2009; Reyes et al. 2012; Cook et al. 2013). Mycoviruses have become an important research subject, particularly since Cryphonectria Yushanjiang Maimaiti and Lei Ding contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42161-020-00607-1) contains supplementary material, which is available to authorized users. * Yushanjiang Maimaiti [email protected] * Wei Chen [email protected] 1
Key laboratory of Integrated Management of Harmful Crop Vermin of China North-western Oasis, ministry of Agricukture, Research Institute of plant protection Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 83009, China
2
College of Life Science, Shanxi Normal University, Linfen 041000, Shanxi, China
3
State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
hypovirus 1 (CHV1) was successfully used to control chest blight disease in Europe (Ghabrial et al. 2015). The discovery of novel mycoviruses is important not only for understanding virus ecology and evolution, but also for the control of plant diseases (Dalman et al. 2013; Persoons et al. 2014). High throughout sequencing (HTS) technologies and bioinformatics are widely used to enhance the study of mycoviruses (Garg et al. 2011; Su et al. 2011; Brautigam et al. 2011; Brautigam et al. 2011; Adams et al. 2009; Altschul et al. 1990)
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