Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a
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ORIGINAL ARTICLE
Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033 Shijin Li1,2 · Zimiao Jia1,3 · Ke Wang1,2 · Lipu Du1 · Hongjie Li1 · Zhishan Lin1,2 · Xingguo Ye1,3 Received: 5 November 2019 / Accepted: 8 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Three genes designated DvLox, Pm21#4, and Pm21#4-H identified in a wheat–Dasypyrum villosum#4 T6V#4S·6DL translocation line Pm97033 conferred wheat for powdery mildew resistance. Abstract Powdery mildew (PM) caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most devastating diseases in wheat. To date, only a few genes conferring resistance to wheat PM are cloned. Dasypyrum villosum is a wild relative of wheat, which provides Pm21 conferring wheat immunity to PM. In this study, we obtained many differentially expressed genes (DEGs) from a wheat–D. villosum#4 T6V#4S·6DL translocation line Pm97033 using RNA-sequencing. Among them, 7 DEGs associated with pathogen resistance were up-regulated in front of Bgt infection. Virus-induced gene silencing and transformation assays demonstrated that two of them, DvLox and Pm21#4 encoding a lipoxygenase and a encoding coiled-coil/nucleotide-binding site/leucine-rich repeat resistance protein, conferred wheat PM resistance. The transgenic wheat plants expressing DvLox enhanced PM resistance, and the transgenic wheat plants expressing Pm21#4 showed PM immunity. The Pm21#4-silenced Pm97033 plants by the cluster regularly interspaced short palindromic repeats–associated endonuclease (CRISPR/Cas9) system were susceptible to PM. Thus, Pm21#4 is a key gene contributing PM immune resistance in Pm97033. Constitutively expression of Pm21#4-H, which is silenced in Pm97033 and D. villosum#4, endowed a PM-susceptible wheat variety Fielder with PM immune resistance.
Introduction
Shijin Li and Zimiao Jia have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03655-4) contains supplementary material, which is available to authorized users. * Zhishan Lin [email protected] * Xingguo Ye [email protected] 1
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China
3
Key Laboratory of Ministry of Agriculture and Rural Affairs of China for Biology and Genetic Breeding of Triticeae Crops, Beijing 100081, China
Wheat (Triticum aestivum L.), as one of the important food crops in the world, plays an essential role in human-related socioeconomic development and food production (Liu et al. 2009; Foley et al. 2011; Wen et al. 2013). Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), is a globally destructive disease. Growing wheat varieties with strong PM resistance is an ideal strategy to reduce the yield losses of wheat due to occurrence of PM. Ge
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