Characterization of Pm68 , a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796
- PDF / 3,118,809 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 202 Views
ORIGINAL ARTICLE
Characterization of Pm68, a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796 Huagang He1,2,6 · Renkang Liu1 · Pengtao Ma3 · Haonan Du1 · Huanhuan Zhang2 · Qiuhong Wu4 · Lijun Yang5 · Shuangjun Gong5 · Tianlei Liu1 · Naxin Huo6 · Yong Q. Gu6 · Shanying Zhu1,2,6 Received: 21 June 2020 / Accepted: 4 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message New powdery mildew resistance gene Pm68 was found in the terminal region of chromosome 2BS of Greek durum wheat TRI 1796. The co-segregated molecular markers could be used for MAS. Abstract Durum wheat (Triticum turgidum L. var. durum Desf.) is not only an important cereal crop for pasta making, but also a genetic resource for common wheat improvement. In the present study, a Greek durum wheat TRI 1796 was found to confer high resistance to all 22 tested isolates of Blumeria graminis f. sp. tritici (Bgt). Inheritance study on the F1 plants and the F2 population derived from the cross TRI 1796/PI 584832 revealed that the resistance in TRI 1796 was controlled by a single dominant gene, herein designated Pm68. Using the bulked segregant RNA-Seq (BSR-Seq) analysis combined with molecular analysis, Pm68 was mapped to the terminal part of the short arm of chromosome 2B and flanked by markers Xdw04 and Xdw12/Xdw13 with genetic distances of 0.22 cM each. According to the reference genome of durum wheat cv. Svevo, the corresponding physical region spanned the Pm68 locus was about 1.78-Mb, in which a number of disease resistance-related genes were annotated. This study reports the new powdery mildew resistance gene Pm68 that would be a valuable resource for improvement of both common wheat and durum wheat. The co-segregated markers (Xdw05–Xdw11) developed here would be useful tools for marker-assisted selection (MAS) in breeding.
Introduction
Communicated by Evans Lagudah. Huagang He, Renkang Liu and Pengtao Ma have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03681-2) contains supplementary material, which is available to authorized users.
Wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is one of the most widely cultivated cereal crops in the world, which provides about 18% of daily dietary calories consumed by humans (Tan et al. 2018; Li et al. 2019). Wheat production is greatly threatened by powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt) that has complex and variable virulence structures in natural populations (Wicker et al. 2013). Exploiting and utilizing broad-spectrum powdery mildew resistance (Pm) genes are important and
* Huagang He [email protected]
3
College of Life Sciences, Yantai University, Yantai 264005, China
* Yong Q. Gu [email protected]
4
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
5
Inst
Data Loading...