Identification and Characterization of circRNAs in the Developing Stem Cambium of Poplar Seedlings
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OMICS. TRANSCRIPTOMICS UDC 577.21
Identification and Characterization of circRNAs in the Developing Stem Cambium of Poplar Seedlings W. Q. Zhenga, b, Y. Zhanga, b, B. Chena, b, M. Weic, X. W. Wanga, b, and L. Dua, b, * aBeijing Advanced Innovation Center of Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, 100083 China b
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083 China cCOFCO Nutrition and Health Research Institute, Beijing, 102209 China *e-mail: [email protected] Received January 2, 2020; revised February 27, 2020; accepted March 5, 2020
Abstract—Non-coding RNAs are a class of RNAs with multiple roles in plant life. Covalently closed circular RNA molecules (circRNAs) have been recently shown to be a group of RNA isoforms that show widespread tissue-specific expression in plants, often cooperating with the corresponding linear mRNAs to regulate gene function. However, no previous study of poplar has identified circRNAs in the cambium and determined their potential roles in the cambium or xylem development. In the present study, we sequenced RNAs in the cambium of poplar seedlings at two developmental stages, and identified and characterized 4912 circRNAs. Alternative back-splicing circularization events for 87 genes were identified among the circRNAs derived from different chromosomes. A total of 1138 circRNAs originated from 928 host genes, which were classified among the three major functional categories by GO analysis. Thirty-nine circRNAs were differentially expressed between cambium samples of stems at two developmental stages. Twenty-four DEcircRNAs interacted with 98 miRNAs as targets, of which some were associated with cambium growth and development. The results suggest that circRNAs play important roles in the cambium in relation to the regulation of stem growth and development in poplar seedlings. Keywords: poplar, circRNA, cambium, miRNA, differential expression, GO analysis DOI: 10.1134/S0026893320050131
INTRODUCTION The vascular system, also termed the vascular tissue system, comprises all vascular tissues in the plant. This tissue system, which is a product of plant adaptation to the terrestrial environment, penetrates the entire plant and is involved in support as well as the transportation and consolidation of substances in the plant body [1, 2]. The vascular system can be divided into the primary and secondary vascular systems on the basis of the developmental sequence and composition of the vascular system. The primary vascular system is present predominantly in primary mature tissues, whereas the secondary vascular system is the vascular tissue present in the secondary mature tissues. The vascular system consists largely of cambium, xylem, and phloem. The vascular cambium is located between the xylem and phloem. Through the division of cambial cells, new xylem and phloem (secondary xylem and secondary phloem) are produced, resulting in diameter growth of the stems and roots. Fusion of the fascicular cambium and interfasci
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