Identification and characterization of Ralstonia spp. causing bacterial wilt disease of vegetables in Mali
- PDF / 630,791 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 56 Downloads / 189 Views
ORIGINAL PAPER
Identification and characterization of Ralstonia spp. causing bacterial wilt disease of vegetables in Mali Wubetu Bihon 1 & Jaw-Rong Chen 2 & Lawrence Kenyon 2 Received: 21 May 2020 / Accepted: 25 July 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract Ralstonia solanacearum species complex is the most destructive and economically important bacterial pathogen of many plant species around the world. It is a particular constraint to the production of the solanaceous vegetables such as tomato, chilli pepper and African eggplant in West Africa. Its broad host range, ability to survive in the soil for long periods and ability to sustain latent infection, make it difficult to control. Identification, characterization and mapping the distribution of the causal Ralstonia spp. strains are necessary to help design effective control strategies. Wilted tomato, African eggplant and pepper plant samples were collected from fields in different regions of Mali. The causal bacterial strains were isolated from the samples and all were identified as Ralstonia pseudosolanacearum. Multiplex PCR using four phylotype specific primer pairs identified the presence of phylotype I strains distributed across all the vegetable production areas whereas phylotype III strains were limited to areas of Sikasso, Koulikoro and Segou to the south and east of Bamako city. Phylogenetic analysis of part of the conserved endoglucanase virulence gene sequences revealed four sequevars with most phylotype I strains identified as sequevars 46 and 31 with only a few as sequevar 14 and one sequevar 18. The phylotype III strains were all in the sequevar 23–48 group. Pathogenicity testing of a selected subset of the strains isolated in Mali showed them all to be pathogenic to the susceptible tomato cultivar Roma. To our knowledge, this is the first study on the molecular diversity within the Ralstonia spp. complex in Mali. This information is important for developing wilt management strategies including breeding for resistance and specifying quarantine restrictions. Keywords Ralstonia solanacearum . Bacterial wilt . Phylotype . Distribution
Introduction Bacterial wilt (BW) caused by soil-borne bacteria of the Ralstonia solanacearum species complex (RSSC) occurs throughout the tropics, sub-tropics and warm temperate regions of the World. More than 250 plant species in more than 50 botanical families can be affected by bacterial wilt Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42161-020-00631-1) contains supplementary material, which is available to authorized users. * Wubetu Bihon [email protected] 1
World Vegetable Center, West and Central Africa, Samanko Research Station, BP 320 Bamako, Mali
2
World Vegetable Center, P. O. Box 42, Shanhua, Tainan 74199, Taiwan
(Wicker et al. 2007; Prior et al. 2016) and the economically important solanaceous crop species potato, tomato, eggplant and chili pepper are particularly susceptible. BW disease is characterized by a su
Data Loading...
