Sequevar distribution of Ralstonia spp. in Solanaceae in the semiarid climate of the Pernambuco state, Brazil
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Sequevar distribution of Ralstonia spp. in Solanaceae in the semiarid climate of the Pernambuco state, Brazil Greecy Mirian R. Albuquerque & Adriano M. F. Silva & Jéssica R. Silva & Edilaine A. Melo & Rosa L. R. Mariano & Maria Cristina Lemos & Edinardo Ferraz & Elineide B. Souza
Accepted: 7 October 2020 # Koninklijke Nederlandse Planteziektenkundige Vereniging 2020
Abstract The phylogenetic relationships and distribution of Ralstonia pseudosolanacearum and Ralstonia solanacearum sequevars were analyzed in 108 strains of Solanaceae in three mesoregions of the Pernambuco State, Brazil. These mesoregions are characterized by a semiarid climate and account for 88.20% of the area of the state. Ralstonia pseudosolanacearum (phylotype I, biovar 3) was predominant in Agreste, and R. solanacearum (phylotype II, biovar 1) was predominant in São Francisco and Sertão. Two haplotypes of R. pseudosolanacearum were identified by analysis of the haplotype network of the partial egl gene sequences and were phylogenetically related to sequevars I-17 and I-18. Five haplotypes of R. solanacearum were identified; two haplotypes were related to sequevar IIA-50,
Supplementary Information The online version of this article (https://doi.org/10.1007/s10658-020-02132-4) contains supplementary material, which is available to authorized users. (*) : A. M. F. Silva : J. R. Silva :
G. M. R. Albuquerque E. A. Melo : R. L. R. Mariano Department of Agronomy, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Recife, PE 52171-900, Brazil e-mail: [email protected]
M. C. Lemos : E. Ferraz IPA- Instituto Agronômico de Pernambuco, Av. Gen. San Martin, Recife, PE 50761-000, Brazil E. B. Souza Department of Biology, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Recife, PE 52171-900, Brazil
and three haplotypes were related to new sequevars IIA61, IIA-62, and IIA-63. In Brazil, sequevars I-17, IIA61, IIA-62, and IIA-63 were detected exclusively in the semiarid climate of Pernambuco, unlike sequevars I-18 and IIA-50, which were detected in other regions of Brazil. Furthermore, R. pseudosolanacearum (phylotype I) and sequevar IIA-50 were detected in the Sertão mesoregion. Sequevar I-18, IIA-61, and IIA-62 strains were prevalent in the Agreste mesoregion, and sequevars I-17 and IIA-50 were prevalent in the São Francisco mesoregion. The virulence variability of the strains toward tomato was related to species and sequevars. Introduced R. pseudosolanacearum strains of sequevar I-18 had higher estimated virulence compared to that of the native R. solanacearum strains of sequevar IIA-50. This is the first study that analyzes the variability of Ralstonia spp. in the semiarid region of Brazil. Keywords Bacterial wilt . Ralstonia solanacearum . Ralstonia pseudosolanacearum . Tomato . egl gene
The state of Pernambuco, which located in the central eastern part of the northeastern region of Brazil, is geographically divided into five mesoregions that are characterized by tropical and semiarid climates (Pernambu
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