Identification, functional characterization, and estrogen regulation on gonadotropin-releasing hormone in the spotted sc
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Identification, functional characterization, and estrogen regulation on gonadotropin-releasing hormone in the spotted scat, Scatophagus argus Hua-pu Chen & Xue-fan Cui & Yao-rong Wang & Zhi-yuan Li & Chang-xu Tian & Dong-neng Jiang & Chun-Hua Zhu & Yong Zhang & Shui-sheng Li & Guang-li Li
Received: 20 January 2020 / Accepted: 20 May 2020 # Springer Nature B.V. 2020
Abstract Gonadotropin-releasing hormone (GnRH) is a key neuropeptide of the reproductive system. However, little is known about the role of GnRH in the spotted scat (Scatophagus argus). Here, three GnRH subtypes (cGnRH-II, sGnRH, and sbGnRH) were identified in the spotted scat. cGnRH-II and sGnRH were only expressed in the brains and gonads of both male and female fish, exhibiting a tissue-specific expression pattern, while sbGnRH was expressed at different transcription levels in all examined tissues. During ovarian maturation, hypothalamus-associated sbGnRH was upregulated, while the expression of sGnRH was variable and cGnRH-II first increased and then decreased. In vivo experiments showed that sbGnRH significantly promoted the expression of fsh and lh genes in a dosedependent manner and exhibited a desensitization effect
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10695-020-00825-5) contains supplementary material, which is available to authorized users. H.
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