Identification of a small set of genes commonly regulated in rice roots in response to beneficial rhizobacteria
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RESEARCH ARTICLE
Identification of a small set of genes commonly regulated in rice roots in response to beneficial rhizobacteria Marine Valette1 • Marjolaine Rey1 • Jeanne Dore´1 • Florence Gerin1 Florence Wisniewski-Dye´1
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Received: 20 September 2020 / Revised: 6 November 2020 / Accepted: 11 November 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Rhizosphere bacteria, whether phytopathogenic or phytobeneficial, are thought to be perceived by the plant as a threat. Plant Growth-Promoting Rhizobacteria (PGPR), such as many strains of the Azospirillum genus known as the main phytostimulator of cereals, cooperate with host plants and favorably affect their growth and health. An earlier study of rice root transcriptome, undertaken with two rice cultivars and two Azospirillum strains, revealed a strain-dependent response during the riceAzospirillum association and showed that only a few genes, including some implicated in plant defense, were commonly regulated in all tested conditions. Here, a set of genes was selected from previous studies and their expression was monitored by qRT-PCR in rice roots inoculated with ten PGPR strains isolated from various plants and belonging to various genera (Azospirillum, Herbaspirillum, Paraburkholderia). A common expression pattern was highlighted for four genes that are proposed to be markers of the rice-PGPR interaction: two genes involved in diterpenoid phytoalexin biosynthesis (OsDXS3 and OsDTC2) and one coding for an uncharacterized protein (Os02g0582900) were significantly induced by PGPR whereas one defense-related gene encoding a pathogenesisrelated protein (PR1b, Os01g0382000) was significantly repressed. Interestingly, exposure to a rice bacterial
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00911-1) contains supplementary material, which is available to authorized users. & Florence Wisniewski-Dye´ [email protected] 1
Ecologie Microbienne, CNRS UMR-5557, INRAe UMR1418, VetAgroSup, Universite´ de Lyon, Universite´ Lyon1, 16 rue Dubois, 69622 Villeurbanne, France
pathogen also triggered the expression of OsDXS3 while the expression of Os02g0582900 and PR1b was downregulated, suggesting that these genes might play a key role in rice-bacteria interactions. Integration of these results with previous data led us to propose that the jasmonic acid signaling pathway might be triggered in rice roots upon inoculation with PGPR. Keywords Azospirillum Genetic markers Oryza sativa PGPR qRT-PCR Root gene expression Abbreviations IAA Indole acetic acid ISR Induced systemic resistance JA Jasmonic acid MAMP Microbe-associated molecular patterns PGPR Plant Growth-Promoting Rhizobacteria
Introduction The rhizosphere, i.e. soil surrounding plant roots, is characterized by incredible diversity and abundance of microorganisms. Whether phytopathogenic or plant-beneficial, a microorganism is perceived by plants, as a threat and the success of the interaction is conditioned b
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