Identification of oleaginous yeasts that metabolize aromatic compounds
- PDF / 2,782,996 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 67 Downloads / 212 Views
BIOENERGY/BIOFUELS/BIOCHEMICALS - ORIGINAL PAPER
Identification of oleaginous yeasts that metabolize aromatic compounds Allison Yaguchi1 · Nicole Franaszek1 · Kaelyn O’Neill1 · Stephen Lee1 · Irnayuli Sitepu2 · Kyria Boundy‑Mills2 · Mark Blenner1 Received: 13 December 2019 / Accepted: 29 February 2020 © Society for Industrial Microbiology and Biotechnology 2020
Abstract The valorization of lignin is critical for the economic viability of the bioeconomy. Microbial metabolism is advantageous for handling the myriad of aromatic compounds resulting from lignin chemical or enzymatic depolymerization. Coupling aromatic metabolism to fatty acid biosynthesis makes possible the production of biofuels, oleochemicals, and other fine/ bulk chemicals derived from lignin. Our previous work identified Cutaneotrichosporon oleaginosus as a yeast that could accumulate nearly 70% of its dry cell weight as lipids using aromatics as a sole carbon source. Expanding on this, other oleaginous yeast species were investigated for the metabolism of lignin-relevant monoaromatics. Thirty-six oleaginous yeast species from the Phaff yeast collection were screened for growth on several aromatic compounds representing S-, G-, and H- type lignin. The analysis reported in this study suggests that aromatic metabolism is largely segregated to the Cutaenotrichosporon, Trichosporon, and Rhodotorula clades. Each species tested within each clade has different properties with respect to the aromatics metabolized and the concentrations of aromatics tolerated. The combined analysis suggests that Cutaneotrichosporon yeast are the best suited to broad spectrum aromatic metabolism and support its development as a model system for aromatic metabolism in yeast. Keyword Lignin · Oleaginous yeast · Aromatic · Cutaneotrichosporon · Rhodotorula
Introduction The biomass economy has largely focused on the sugars derived from the cellulose and hemicellulose fractions of lignocellulose; however, recent studies by the US Department of Energy show that valorization of lignin is necessary to make biofuels economical [1–3]. A billion-ton biomass economy would produce approximately 225 million tons of lignin by-product [3, 4]. Proposed uses for this lignin Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10295-020-02269-5) contains supplementary material, which is available to authorized users. * Mark Blenner [email protected] 1
Department of Chemical and Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd, Clemson, SC 29634, USA
Phaff Yeast Culture Collection, Food Science and Technology, University of California Davis, One Shields Ave, Davis, CA 95616, USA
2
are largely low-value, including polyurethane products [5], and phenolic adhesives [6], or are niche markets requiring significant lignin purification, such as carbon fibers [7–9]. Several barriers prevent the valorization of lignin, including its heterogenous composition and the resulting heterogeneity of its depolymerized components [10–1
Data Loading...
