Identification of two QTLs for resistance to Fusarium wilt race 1 in lettuce ( Lactuca sativa L.)
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Identification of two QTLs for resistance to Fusarium wilt race 1 in lettuce (Lactuca sativa L.) Kousuke Seki . Kenji Komatsu . Masahiro Hiraga . Keisuke Tanaka Yuichi Uno . Hideo Matsumura
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Received: 12 May 2020 / Accepted: 10 October 2020 Ó Springer Nature B.V. 2020
Abstract Fusarium wilt race 1, caused by Fusarium oxysporum f. sp. lactucae, is one of the main diseases of lettuce in tropical and subtropical regions, including under summer conditions in Japan. Resistant lettuce cultivars have shown symptoms on occasion, which have led breeding programs to focus on the improvement of resistance phenotypes to race 1. Disease assays using Fusarium wilt race 1 were performed, and the lettuce cultivar ‘VI185’ was categorized as highly resistant, which was validated by subsequent Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10681-020-02713-8) contains supplementary material, which is available to authorized users.
population analyses. F2 individuals obtained from a cross between ‘VI185’ (highly resistant) and ‘ShinanoGreen’ (partially resistant) were analyzed, and an F3 population was subjected to an infection assay using the race 1 pathogen. ddRAD-seq analysis revealed two significant QTLs on LG7 (qFOL7.1) and LG8 (qFOL8.1) that accounted for the race 1 resistance phenotype. Furthermore, the loci that conferred elevated resistance originated from ‘VI185’ at both QTLs. Our results provide a foundation for understanding the QTLs for resistance to Fusarium wilt race 1 and provide evidence that genetic diversity is available for enhancing the current resistance in existing lettuce cultivars.
K. Seki (&) Nagano Vegetable and Ornamental Crops Experiment Station, 1066-1 Tokoo, Souga, Shiojiri, Nagano 399-6461, Japan e-mail: [email protected]
Y. Uno Plant Science Division, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan
K. Komatsu Department of Bioresource Development, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa 243-0034, Japan
H. Matsumura Gene Research Center, Shinshu University, 3-15-1 Tsuneta, Ueda, Nagano 386-8567, Japan
M. Hiraga Nagano Fruit Tree Experiment Station, 492 Ogawara, Suzaka, Nagano 382-0072, Japan K. Tanaka NODAI Genome Research Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan
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Keywords Fusarium wilt race 1 Disease resistance Lettuce breeding RAD-seq QTL
Introduction Lettuce, Lactuca sativa L., is a highly popular leafy vegetable, and a common disease of lettuce worldwide is Fusarium wilt, which was first observed in Japan in 1955 (Matuo and SM 1967). Fusarium wilt has subsequently been identified in the United States (Hubbard and Gerik 1993; McCreight et al. 2005), Taiwan (Huang JH 1998), Iran (Millani MJ, Erebarian HR 1999), Portugal (Pasquali et al. 2007), Italy (Pasquali et al. 2005, 2007), and Brazil
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