Immune landscape of periodontitis unveils alterations of infiltrating immunocytes and molecular networks-aggregating int
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Journal of Translational Medicine Open Access
RESEARCH
Immune landscape of periodontitis unveils alterations of infiltrating immunocytes and molecular networks‑aggregating into an interactive web‑tool for periodontitis related immune analysis and visualization Xiaoqi Zhang1, Qingxuan Wang1, Xinyu Yan1, Yue Shan1, Lu Xing1, Minqi Li2, Hu Long1 and Wenli Lai1*
Abstract Background: Immunity reaction plays an essential role in periodontitis progress and we aim to investigate the underlying regulatory network of immune reactions in periodontitis. Methods: CIBERSORT was used to estimate immunocyte fractions in different clinical statuses. Logistic regression was used to assess the immunocyte weight in periodontitis. Immune-related periodontitis subtypes were identified by the Nonnegative Matrix Factorization algorithm. Gene-set enrichment analysis and Gene-set variation analysis were conducted to analyze pathway activities. Immunocytes related gene modules were identified by Weighted gene co-expression network analysis. Results: Altered immunocytes in healthy versus periodontitis, aggressive versus chronic, male versus female and age were identified. Immunocytes enriched in periodontitis were calculated, and their correlation was also explored. Two distinct immune-related periodontitis subtypes were identified and one is characterized by B cell reactions and the other is IL-6 cytokine reactions. 463 statistically significant correlations between 22 immunocytes and pathways were revealed. Immunocytes and clinical phenotypes matched their gene modules, and their functions were annotated. Last, an easy-to-use and user-friendly interactive web-tool were developed for periodontitis related immune analysis and visualization (https://118.24.100.193:3838/tool-PIA/). Conclusions: This study systematically investigated periodontitis immune atlas and caught a glimpse of the underlying mechanism of periodontitis from gene-pathway-immunocyte networks, which can not only inspire researchers but also help them in periodontitis related immune researches. Keywords: Periodontitis, Immune, Immunocyte, Gene, Pathway, Web-tool, Bioinformatics
*Correspondence: [email protected] 1 Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center of Oral Diseases, West China Hospital of Stomatology, Sichuan University, 14, Sect 3, Renmin South Rd, Chengdu 610041, Sichuan, China Full list of author information is available at the end of the article
Background Periodontitis is one of the three most common oral diseases with a high morbidity rate [1]. Epidemiological investigation revealed that periodontitis is the primary cause of tooth loss worldwide [2]. Periodontitis is characterized by the inflammatory destruction of periodontal support tissues, with main clinical manifestations
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