Immunohistochemical basigin expression level in thyroid cancer tissues
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(2020) 18:240
RESEARCH
Open Access
Immunohistochemical basigin expression level in thyroid cancer tissues Wan-Ping Guo1†, Deng Tang1†, Yu-Yan Pang1, Xiao-Jiao Li2, Gang Chen1, Zhi-Guang Huang1, Xiao-Zhun Tang3, Qin-Qiao Lai1, Jin-Yan Gan1, Xiao-Li Huang1, Xiao-Fan Liu1, Zhi-Xiao Wei2* and Wei Ma1*
Abstract Background: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. Methods: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays, and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. Results: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly upregulated in TC cases. Conclusion: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions. Keywords: Basigin, Thyroid cancer, Thyroid papillary carcinoma, Tissue microarray, RNA-sequencing
Background Thyroid cancer (TC) is the most common endocrine malignancy, and its prevalence has increased dramatically in the last few years [1–3]. Histological types of TC include papillary carcinoma (PTC), follicular carcinoma * Correspondence: [email protected]; [email protected] † Wan-Ping Guo and Deng Tang contributed equally to this work. 2 Department of Nuclear Medicine, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning 530021, Guangxi Zhuang Autonomous Region, People’s Republic of China 1 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning 530021, Guangxi Zhuang Autonomous Region, People’s Republic of China Full list of author information is available at the end of the article
(FTC), anaplastic carcinoma (ATC)/undifferentiated thyroid carcinoma(UTC), and medullary carcinoma (MTC) [4], and there have been different mutational profiles in these subtypes. Recent studies have confirmed a few molecular markers that have allowed better understanding of the molecular mechanis
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