In vitro and in vivo anthelmintic study of Sesbania sesban var. bicolor , a traditionally used medicinal plant of Santha
- PDF / 1,268,412 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 105 Downloads / 171 Views
ORIGINAL ARTICLE
In vitro and in vivo anthelmintic study of Sesbania sesban var. bicolor, a traditionally used medicinal plant of Santhal tribe in Assam, India Amar Deep Soren1,2
•
Risa Parkordor Chen1
•
Arun K. Yadav1
Received: 19 March 2020 / Accepted: 1 September 2020 Ó Indian Society for Parasitology 2020
Abstract The leaf decoction of Sesbania sesban var. bicolor is used traditionally by Santhal tribe of Assam, India, for the treatment of intestinal helminthic infections. This study was conducted to evaluate the in vitro and in vivo anthelmintic efficacy of methanolic extract of S. sesban var. bicolor leaves using Hymenolepis diminuta-rat (cestode) and Syphacia obvelata-mice (nematode) as test parasites and models. Praziquantel (PZQ) and albendazole (ABZ) were used as reference drugs. At the highest concentration of 30 mg/ml of the plant extract, H. diminuta and S. obvelata showed mortality at 0.81 ± 0.01 h and 15.17 ± 0.05 h, respectively. The in vivo results substantiated the in vitro findings, and the extract showed a better cestocidal efficacy in a dose-dependent manner, whereby treatment of rats with 400 mg/kg of the plant extract caused 65.10% reduction in eggs per gram (EPG) of faeces and 56% reduction in worm counts. S. obvelata-infected mice treated at the same dose showed 34.32% and 47.08% reduction in EPG and worm counts at necropsy, respectively. The methanolic extract was subjected to bioassayguided fractionation using different solvents and the ethyl acetate fraction proved to be the most active. This active fraction was subjected to column chromatography using varying concentrations of hexane:ethyl acetate. Maximum efficacy was observed in 7:3 hexane:ethyl acetate, where H. diminuta and S. obvelata showed mortality at 3.56 ± 0.12 h and 9.21 ± 0.02 h, respectively. This & Amar Deep Soren [email protected] 1
Department of Zoology, North-Eastern Hill University, Shillong, Meghalaya 793022, India
2
Present Address: Department of Zoology, B. Borooah College, Guwahati, Assam 781007, India
indicates that the isolated fraction contained the active component responsible for its anthelmintic activity, which substantiates the medicinal usage in traditional practice. Keywords Active fraction Anthelmintic Helminthiasis Hymenolepis diminuta Sesbania sesban var. bicolor Syphacia obvelata
Introduction Intestinal helminthiasis, is an important public health problem in tropical and sub-tropical countries. It affects around 1.5 billion people globally (WHO 2015). At present the control of intestinal worms is based on mass drug treatment by two common drugs, albendazole and mebendazole. However, in some areas of the world synthetic medicines are still out of reach and therefore, alternative strategies developed from traditional knowledge to combat such diseases have emerged since times immemorial (Deori and Yadav 2016). Sesbania sesban (L.) Merr. var. bicolor (Wight & Arn.) F. W. Andrews (Fabaceae) (Fig. 1), commonly called as ‘‘Mondormoli’’ in the Santhali language, is widely distributed an
Data Loading...
