In vitro anti-inflammatory, antioxidant and antibacterial activities of the Algerian species Micropus bombycinus
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In vitro anti‑inflammatory, antioxidant and antibacterial activities of the Algerian species Micropus bombycinus Samia Dekkiche1 · Chaima Mouffouk2 · Soumia Mouffouk2 · Hamada Haba2 Received: 19 April 2020 / Accepted: 6 September 2020 © Institute of Korean Medicine, Kyung Hee University 2020
Abstract The present study reports the in vitro pharmacological properties of the methanolic extract obtained from the species Micropus bombycinus Lag. The quantification of total phenolic content was performed using the Folin-Ciocalteu method. The antioxidant activity was evaluated by four different assays. Moreover, the in vitro anti-inflammatory activity was assessed using the albumin denaturation method and the antibacterial activity was estimated by agar disk diffusion assay against ten bacterial strains. The Micropus bombycinus methanolic extract contains several classes of secondary metabolites known for their biological properties. A high content of polyphenols was estimated (115.32 ± 0.47 μg GAE/ mg of plant extract). The MeOH extract possessed strong antioxidant activities in various assays that subsequently correlated with its phenolic content. It had also the ability to inhibit thermally-induced protein denaturation with a percentage of 27.51% at the concentration of 400 μg/mL. Furthermore, this extract showed antibacterial activity against all the tested strains with zones of inhibition ranging from 9 to 28 mm. It can be concluded that Micropus bombycinus could be an interesting source for potential bioactive compounds as inhibitors of pathogenic bacteria, and it helps to cure chronic inflammatory (Rheumatoid arthritis) and oxidative stress-related diseases. Keywords Micropus bombycinus · Anti-inflammatory · Antioxidant · Antibacterial · Polyphenols
Introduction Free radicals are defined as chemical elements with an unpaired electron on the peripheral layer of the molecular skeleton (Halliwell and Gutteridge 2015). In biological organisms, free radicals share the characteristic of having a single electron on oxygen or nitrogen atoms creating reactive oxygen species (ROS) or reactive nitrogen species (RNS). These radicals are generated under environmental stress such as pollution, alcohol consumption or certain medications Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13596-020-00506-x) contains supplementary material, which is available to authorized users. * Hamada Haba [email protected]; hamada.haba@univ‑batna.dz 1
Laboratoire de Biologie Moléculaire et Cellulaire (LBMC), Département de Microbiologie, Faculté Des Sciences, Université Mentouri, Ain Elbye, Constantine 1, Algérie
Laboratoire de Chimie et Chimie de l’Environnement (LCCE), Département de Chimie, Faculté Des Sciences de la Matière, Université Batna 1, 05000 Batna, Algérie
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(Kalam et al. 2015), and as a result of several enzymatic reactions (NADPH oxidase, lipoxygenase, xanthine oxidase) (Phaniendra et al. 2014). Reactive oxygen and nitrogen species are well
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