Evaluation of antibacterial and antioxidant activities of Cissus rotundifolia (Forssk.) leaves extract obtained by ultra
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ORIGINAL PAPER
Evaluation of antibacterial and antioxidant activities of Cissus rotundifolia (Forssk.) leaves extract obtained by ultrasonic-assisted extraction conditions Wedad Q. AL‑Bukhaiti1 · Anwar Noman1,2 · Qais A. AL‑Maktary1 · Abdelmoneim H. Ali1 · Hongxin Wang1 Received: 26 March 2020 / Accepted: 8 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this study, the antibacterial and antioxidant activities of Cissus rotundifolia leaves extract were evaluated. The active compounds of plant leaves were extracted by using ultrasonic-assisted extraction conditions. Yield, total phenolic compounds and antioxidant activities were determined. In addition to six strains of gram-positive and negative bacteria were used for testing antibacterial activities of the plants’ extracts. The obtained results demonstrated that extraction of active compounds by using ultrasonic-assisted extraction was more efficient. The dry yield and total phenol compounds of extract were 29.97% and 82.87 mg GAE/g DE, respectively. The antibacterial activities of the extracts were tested against two types of bacteria, Gram-positive strains: Bacillus subtilis, Staphylococcus aureus, and Streptococcus agalactiae, and Gram-negative strains: Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa. The results showed that leaves extract was more effective against Gram-negative bacterial strains. The concentrations of minimum inhibitory and minimum bactericidal of the leaves extract against S. typhimurium, E. coli and P. aeruginosa (Gram-negative bacteria) were at concentrations of 10, 20 and 20 mg/mL, respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothioline6-sulphonic acid)-diammonium salt (ABTS) scavenging activities at a concentration of 2 mg/mL were 90.94% and 98.20%, respectively, while the I C50 was achieved at concentrations of 0.475 and 0.790 mg/mL, respectively. The leaves extract has high antibacterial and antioxidant activities; therefore, it could be a rich source of natural antibacterial and antioxidants for several food and therapeutic applications. Keywords Cissus rotundifolia · Total phenolic compounds · Antibacterial · Bacterial strains · Antioxidant activity
Introduction Wild plants have returned to the forefront of attention recently because of the results of scientific research confirming their high nutritional value and their content of antioxidant and antimicrobial compounds. Wild vegetables are the cheapest source of protein, vitamins, minerals, and essential * Wedad Q. AL‑Bukhaiti [email protected] * Hongxin Wang [email protected] 1
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Department of Agricultural Engineering, Faculty of Agriculture, Sana’a University, Sana’a, Yemen
2
amino acids in the food system of many people [1]. In addition the consumption of wild vegetables plays an important role as food supplements contrib
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