Increased P3HB Accumulation Capacity of Methylorubrum sp. in Response to Discontinuous Methanol Addition
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Increased P3HB Accumulation Capacity of Methylorubrum sp. in Response to Discontinuous Methanol Addition Letícia Oliveira Bispo Cardoso 1,2,3 & Bruno Karolski 2,3 & Louise Hase Gracioso 1,2,3 & Claudio Augusto Oller do Nascimento 1,2,3 & Elen Aquino Perpetuo 2,3,4 Received: 24 April 2020 / Accepted: 22 June 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
An alternative for non-biodegradable oil-based plastics has been the focus of many researchers throughout the years. Polyhydroxyalkanoates (PHAs) are potential substitutes due to their biodegradable characteristic and diversity of monomers that allow different biopolymer compositions and physical–chemical properties suitable for a variety of applications. The most well-known biopolymer from this class, poly(3-hydroxybutyrate) (P3HB), is already produced industrially, but its final price cannot compete with the oilbased plastics. As a low-volume high-value bioproduct, P3HB must be produced through a cheap and abundant feedstock, with high productivity and a feasible purity process in order to become an economically attractive bioproduct. In this scenario, we report a methylotrophic strain isolated from an estuarine contaminated site identified as Methylorubrum sp. highly tolerant to methanol and with great accumulation capacity of 60% (CDW) in 48 h through a simple strategy of batch fermentation with discontinuous methanol addition that could help lower P3HB’s processing costs and final price. Keywords Methanol . Methylotrophs . Polyhydroxyalkanoates . Polyhydroxybutyrate . Biopolymers . Bioproducts
* Letícia Oliveira Bispo Cardoso [email protected]
1
Chemical Engineering Department (POLI-USP), University of São Paulo, Sao Paulo, Brazil
2
Research Centre for Gas Innovation (RCGI-POLI-USP), University of São Paulo, Sao Paulo, Brazil
3
Environmental Research and Education Center (CEPEMA-POLI-USP), University of São Paulo, Sao Paulo, Brazil
4
Institute of Marine Sciences (IMar-UNIFESP), Federal University of São Paulo, Sao Paulo, Brazil
Applied Biochemistry and Biotechnology
Introduction Worldwide plastic production reached 359 million tons in 2018 [1]. Considering that oil-based plastics are non-biodegradable, which only 9% of it are recycled and the remaining is often discarded inappropriately, plastics represent a major threat of our century [2]. Yet, our society is highly dependent on plastics. We use it for many applications, from food packaging and daily tools to automobile parts and the construction sector [1]. Since plastics have become such a versatile material that can be used in uncountable goods, its production shall continue to increase but with evolving technology, we can introduce bioplastics in the market; biodegradable options such as polyhydroxyalkanoates could change the image of plastics as pollution agents. Polyhydroxyalkanoates (PHAs) are potential substitutes to oil-based plastics since they present similar properties and can be synthesized and decomposed by the same microorganisms, which are
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