Increased PELP1 expression in rat periodontal ligament tissue in response to estrogens treatment
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ORIGINAL PAPER
Increased PELP1 expression in rat periodontal ligament tissue in response to estrogens treatment Jing Wang • Qiang Zhu • Shujun Song • Jun Dong • Lixin Shi • Ran Tao • Yin Ding Baofa Hong
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Received: 30 November 2012 / Accepted: 4 February 2013 / Published online: 24 February 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Estrogens and their receptors are important factors involved in periodontal ligament (PDL) tissue health. As a regulator of estrogen receptors (ER), the proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) may play a role in alveolar bone formation and PDL homeostasis. The aim of the present study was to observe PELP1 expression in rat PDL tissue during estrogen levels manipulations. Twentyone 8-week old normal female Sprague–Dawley rats were randomly divided into three equal groups: sham-operated controls, ovariectomized (OVX) group, and OVX given 17 b-estradiol intraperitoneally (OVX ? E2) for 16 weeks. PELP1 expression was down-regulated in the OVX group and was up-regulated in the OVX ? E2 group. Periodontal ligament fibroblast cells (PDLFCs) were isolated from PDL tissue, and characterized by immunohistochemical staining. Estradiol treatment of PDLFCs induced PELP1 protein level
Jing Wang and Qiang Zhu contributed equally to this work. J. Wang Y. Ding (&) Department of Orthodontics, College of Stomatology, Forth Military Medical University, Xin Cheng District, Xi’an 710032, China e-mail: [email protected]; [email protected] Q. Zhu J. Dong L. Shi B. Hong (&) Department of Urological, General Hospital of People’s Liberation Army, Hai Dian District, Beijing 100853, China e-mail: [email protected] S. Song Department of Pathology and Experimental Medicine, 306 Hospital of PLA, Chao Yang District, Beijing 100101, China R. Tao Department of Plastic Surgery, General Hospital of People’s Liberation Army, Hai Dian District, Beijing 100853, China
compared to untreated cells. PELP1 mRNA expression in estradiol-treated cells was relatively low at the beginning of treatment and then steadily increased from hour 4. In conclusion, results indicate that PELP1 is expressed in rat PDL tissue and PDLFCs, and that its expression is up-regulated during estrogen treatment. Keywords Estrogen PELP1 Ovariectomized rats Periodontal ligament
Introduction Decreased estrogens levels are believed to be the major cause of many age-related diseases, such as cardiovascular diseases, and periodontal diseases, especially among postmenopausal women (Abujazia et al. 2012; Toran-Allerand 2004; Smuc et al. 2009; Bord et al. 2003; Dobrzycka et al. 2003). This decrease in estrogens levels after menopause leads to micro-damages to the periodontal ligament (PDL) tissue. PDL tissue expresses estrogen receptors (ER) a and b and is then an important target for estrogens. It is also a non-mineralized connective tissue, lying between alveolar bone and cementum, and it is playing a vital role in maintaining periodontal tissues homeostasis (Wang et al. 2006; Seo et al. 2004)
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