Inhibition of Quorum Sensing, Motility and Biofilm Formation of Pseudomonas aeruginosa by Copper Oxide Nanostructures
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ORIGINAL PAPER
Inhibition of Quorum Sensing, Motility and Biofilm Formation of Pseudomonas aeruginosa by Copper Oxide Nanostructures Dnyanada G. Desai1 • H. Swarali2 • Govinda R. Navale2 • A. Prabhune2 • Dattatray J. Late3 Mahesh S. Dharne2 • Pravin S. Walke1
•
Received: 23 April 2020 / Accepted: 11 October 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Quorum sensing (QS) is the communication between bacterial cells governed by their population density and regulated by the genes controlling virulence factors and biofilm formation. Multiple mechanisms of biofilms are resistive to antimicrobial chemotherapy; therefore novel strategies are required to overcome its limitations. Here, we report the effect of various copper oxide nanostructures (CuO-NSs) on quorum sensing inhibition. The two-dimensional CuO-NSs such as interlaced nanodiscs, nanodiscs and leaf-shaped nanosheets are prepared via a simple chemical method. The Quorum sensing inhibition (QSI) activity of all the CuO-NS are examined using reporter strain Chromobacterium violaceum CV026 and Escherichia coli pSB1142. We found that the CuO-interlaced nanodisc structures exhibit better QSI activity than nanodiscs and leaf-shaped sheets. The interlaced nanodisc structures are inhibited various long-chain N-acyl homoserine lactones (AHLs) mediated QS individually and confirmed by other QS-associated phenomena for Pseudomonas aeruginosa, including biofilm inhibition, inhibition of virulence factors such as pyocyanin, protease production and swarming motility. Thus QSI activity of CuO-NSs is solely dependent on specific shape offering large surface area and more active sites. The CuO-NS is effective quorum sensing inhibitors, which has potential clinical applications in the management of P. aeruginosa associated infections. Keywords Nanostructures P. aeruginosa Biofilm Quorum sensing Virulence factors
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10876-020-01916-2) contains supplementary material, which is available to authorized users. & Mahesh S. Dharne [email protected] & Pravin S. Walke [email protected] 1
National Centre for Nanoscience and Nanotechnology, University of Mumbai, Kalina, Santacruz (E), Mumbai 400098, India
2
NCIM Resource Centre, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, Maharashtra 411008, India
3
Centre for Nanoscience & Nanotechnology, Amity University, Mumbai 410206, India
Nowadays, controlling many diseases across the globe has become a major concern due to the extensive development of drug resistance in microbes [1]. The resistance mechanism includes the production of drug-inactivating enzymes, alteration of drug targets, reformed drug uptake or efflux, and biofilm formation. However, the biofilm formation ability of bacteria is a major cause of antibiotic resistance as bacteria become more resistant to antibiotics under the protective layer of biofilm.
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