Acyl homoserine lactone-based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditi
- PDF / 2,303,850 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 61 Downloads / 183 Views
ORIGINAL PAPER
Acyl homoserine lactone‑based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions Felipe Alves de Almeida1 · Natan de Jesus Pimentel‑Filho2 · Uelinton Manoel Pinto3 · Hilário Cuquetto Mantovani1 · Leandro Licursi de Oliveira4 · Maria Cristina Dantas Vanetti1
Received: 15 February 2016 / Revised: 7 October 2016 / Accepted: 22 October 2016 © Springer-Verlag Berlin Heidelberg 2016
Abstract Quorum sensing regulates a variety of phenotypes in bacteria including the production of virulence factors. Salmonella spp. have quorum sensing systems mediated by three autoinducers (AI-1, AI-2, and AI-3). The AI-1-mediated system is incomplete in that the bacterium relies on the synthesis of signaling molecules by other microorganisms. This study aimed to evaluate the influence of the AI-1 N-dodecanoyl-DL-homoserine lactone (C12HSL) on the growth, motility, adhesion, and biofilm formation of Salmonella enterica serovar Enteritidis PT4 578 on a polystyrene surface. Experiments were conducted at 37 °C in anaerobic tryptone soy broth supplemented with C12-HSL and/or a mixture of four synthetic furanones, at the concentration of 50 nM each. The planktonic growth, adhesion, swarming, and twitching motility were not altered in the presence of C12-HSL and/or furanones under anaerobic conditions. However, C12-HSL induced biofilm
formation after 36 h of cultivation as determined by quantification of biofilm formation, by enumeration of adhered cells to polystyrene coupons, and finally by imaging the presence of multilayered cells on an epifluorescence microscope. When furanones were present in the medium, an antagonistic effect against C12-HSL on the biofilm development was observed. The results demonstrate an induction of biofilm formation in Salmonella Enteritidis by AI-1 under anaerobic conditions. Considering that Salmonella does not produce AI-1 but respond to it, C12-HSL synthesized by other bacterial species could trigger biofilm formation by this pathogen in conditions that are relevant for its pathogenesis. Keywords Adhesion · Autoinducer · Biofilm · Cellular communication · Furanone
Introduction Communicated by Jorge Membrillo-Hernández. Electronic supplementary material The online version of this article (doi:10.1007/s00203-016-1313-6) contains supplementary material, which is available to authorized users. * Maria Cristina Dantas Vanetti [email protected] 1
Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570‑900, Brazil
2
Center of Nature Science, Universidade Federal de São Carlos, Buri, São Paulo, Brazil
3
Department of Food and Experimental Nutrition, Food Research Center, Universidade de São Paulo, São Paulo, São Paulo, Brazil
4
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil
Salmonella is one of the most common foodborne pathogens frequently associated with illness, hospitalization, and deaths worldwide (Finstad et al. 2012; Nunes et al. 2013; Chironna et al. 2014). I
Data Loading...
